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Home / Primary Antibodies

beta-Actin Antibody

SKU : BHA12905571

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	T0022
Alternative Names	ACTB ; Actin ; cytoplasmic 1 ; Beta-actin ; Beta actin ; BRWS1 ; Actin ; beta ; Beta cytoskeletal actin ; PS1TP5-binding protein 1 ; PS1TP5BP1 ;
Categories	Primary Antibodies
Clonality	monoclonal
Description	beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Immunogen	Full-length beta-Actin protein of human.
Molecular Weight	Observed Mol.Wt.: 43KD. Predicted Mol.Wt.: 42kDa.
Reactivity	Guinea pig, Zebrafish, Chicken, Hamster, Monkey, Plants, Rabbit, Human, Mouse, Sheep, Fish, Goat, Rice, Cow, Dog, Pig, Rat
Specificity	beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.
Subcellular Location	Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Uniprot

P60709

Gene Id

60

Sequence

10	20	30	40	50
MDDDIAALVV	DNGSGMCKAG	FAGDDAPRAV	FPSIVGRPRH	QGVMVGMGQK
60	70	80	90	100
DSYVGDEAQS	KRGILTLKYP	IEHGIVTNWD	DMEKIWHHTF	YNELRVAPEE
110	120	130	140	150
HPVLLTEAPL	NPKANREKMT	QIMFETFNTP	AMYVAIQAVL	SLYASGRTTG
160	170	180	190	200
IVMDSGDGVT	HTVPIYEGYA	LPHAILRLDL	AGRDLTDYLM	KILTERGYSF
210	220	230	240	250
TTTAEREIVR	DIKEKLCYVA	LDFEQEMATA	ASSSSLEKSY	ELPDGQVITI
260	270	280	290	300
GNERFRCPEA	LFQPSFLGME	SCGIHETTFN	SIMKCDVDIR	KDLYANTVLS
310	320	330	340	350
GGTTMYPGIA	DRMQKEITAL	APSTMKIKII	APPERKYSVW	IGGSILASLS
360	370
TFQQMWISKQ	EYDESGPSIV	HRKCF

Concentration	1mg/ml
Format	Liquid
Purification	Affinity-chromatography.
Storage Condition	Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IF/ICC, ELISA, IHC, WB
Dilution	WB: 1:3000~1:10000, IHC: 1:200, IF/ICC: 1:200, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12905571	T0022	100ul	$250		Add to Cart
BHA12905571	T0022	1ml	$1200		Add to Cart
BHA12905571	T0022	200ul	$350		Add to Cart
BHA12905571	T0022	50ul	$150		Add to Cart

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