This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.
Uniprot
P10415
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the recombinant Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.