This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Recombinant full length human protein was used as the immunogen for this recombinant Bcl-2 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human
Note
The optimal dilution of the recombinant Bcl-2 antibody for each application should be determined by the researcher.
Uniprot
P10415
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant Bcl-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT