BspQI GMP grade (10 U/uL)

Mfr.No.

10664ES76, 10664ES92, 10664ES86

Description

This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.

Feature

Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GCTCTTC (1/4)
Digestion of DNA to obtain specific sticky ends
Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, RNases

Application

Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
Linearize plasmid template before in vitro transcription
Restriction digest

Specification

Expression Host	Recombinant E. coli with BspQI gene
Reaction Temperature	50℃
Storage Buffer	20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol
Unit Definition	1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system

Components

Components No.	Name	10664ES76 (500 U)	10664ES86 (2,500 U)	10664ES92 (10 KU)	10664ES98 (100 KU)
10664	BspQI GMP-grade (10 U/μL)	50 μL	250 μL	1 mL	10 mL

Storage	Stored at -15℃ ~ -25℃ for one year.
Shipping	The product is shipped with dry ice.
Format	Endonuclease

Protocals And Manuals	Safety Data Sheet Manual

No Description Available.

The performance of YEASEN BspQI is superior. In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded. M: DNA marker C: The control group without BspQI treatment

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Combination SKU	Supplier No.	Size	Your price
BHZ20800005-1	10664ES76	500 U	$105	Add to Cart
BHZ20800005-2	10664ES92	10 KU	$1275	Add to Cart
BHZ20800005-3	10664ES86	2500 U	$325	Add to Cart

BspQI GMP grade (10 U/uL)

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