Integrated AAV Offerings: From Cutting-Edge Products to Advanced Custom Services

Key Features of the ATHENA II Platform

Random Peptide Insert Library:

The ATHENA II platform utilizes random peptide insertion into variable sites of the AAV capsid protein to create a highly diverse library. These libraries allow for the identification of novel capsids that exhibit unique properties, such as altered tissue tropism, enhanced transduction efficiency, and lower immune responses, which are critical for gene therapy applications.

Premade AAV Capsid Libraries (II)

 AAVnerGene offer premade AAV Capsid Libraries (II) where the CAP gene with random peptides is driven by a CAG/P40 hybrid promoter. The P40 promoter is used during AAV production, while the CAG promoter is used for CAP expression in target cells or tissues. Most premade libraries insert peptides at the VR-VIII and VR-IV regions, which are known for their roles in capsid function.
 

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SKU	Name	Insert Region	Insert Length	Library Amount
DB005-AAV1	AAV1 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAV2	AAV2 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AV3B	AAV3B Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAV5	AAV5 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAV6	AAV6 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAV7	AAV7 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAV8	AAV8 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAV9	AAV9 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAVrh.10	AAVrh.10 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul
DB005-AAVrh.74	AAVrh.74 Capsid Libraries	VR-IV VR-VII	NNK7 NNK8 NNK9	100ul 200ul 500ul 1000ul

Notes: 

1.         Complexity. The complexity was calculated according the numbers of clones after electroporation.
2.          AAV library production. The AAV library plasmid was transfected as 200 Copies/cells together with our mini-pHelper-Rep.
3.          AAV purification: two rounds of CsCl ultracentrifuge.
4.          For the premade AAV capsid library, we only provide AAV virus but not the plasmid.

Custom AAV Capsid Library Design and Production

Custom ATHENA II libraries allow researchers to tailor their AAV capsid libraries by selecting library templates, the length of random peptides, insertion sites, and library complexity. The process involves designing appropriate expression strategies, selecting AAV serotypes, identifying variable regions, and determining peptide insertion or replacement strategies.
 

AAV Capsid Library Construction Services​

We offer generation of random peptide libraries from modified AAV capsid plasmids. The price is dependent on the efficiency and complexity requested. Each library includes amplification of 1mg of endotoxin free plasmid.

Library complexity	Price	Turnaround Time
1.00E+07	$12,800	3-4 weeks
1.00E+08	$21,800	3-4 weeks
1.00E+09	$68,000	4-5 weeks
Higher	Please contact us to discuss

 

AAV Capsid Library Production Services

We offer packaging of random peptide libraries from modified AAV capsid plasmid. Our pricing is dependent on quantity and transfection conditions. Pricing increases with lower copies per cell.

Scale	Packaging Conditions	Price	Turnaroud Time
2.00E+12	1000 copies/cell	$11,800	2-3 weeks
	200 copies/cell	$19,800	2-3 weeks
	100 copies/cell	$29,800	2-3 weeks
	<50 copies/cell	Please call to discuss
2.00E+13	1000 copies/cell	$48,800	3-4 weeks
	200 copies/cell	$98,000	3-4 weeks
	100 copies/cell	$148,800	3-4 weeks
	<50 copies/cell	Please call to discuss
2.00E+14	1000 copies/cell	Please call to discuss
	200 copies/cell	Please call to discuss
	100 copies/cell	Please call to discuss
	<50 copies/cell	Please call to discuss

AAV Capsid Library Evolution

We can do AAV capsid evolution both in vitro (primary cells and cell lines) and in vivo (mouse, rat, pig and NHP). Please discuss with our technical support for your screening needs.

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