Loading ....
Integrated AAV Offerings: From Cutting-Edge Products to Advanced Custom Services
Overcoming AAV Technology Challenges with Expertly Tailored Vector Design and Production
ATHENA II AAV Capsid Platform
The ATHENA-II platform is a state-of-the-art random peptide (RP) insertion AAV capsid library system designed to develop novel AAV capsids with tissue-specific tropism. In these AAV capsid libraries, random peptide sequences are inserted into specific variable regions (VRs) of the AAV capsid protein. These modifications can significantly alter the capsid's properties, such as its tissue tropism, transduction efficiency, and immunogenicity, making them more suitable for various gene therapy applications. By screening these libraries, researchers can discover and select novel AAV capsids with enhanced properties that are ideal for specific therapeutic needs.
Biohippo, in partnership with AAVnerGene, offers high-complexity AAV capsid libraries containing over 1 billion unique variants, providing a vast resource for exploring new capsid functionalities. We also provide custom AAV capsid library construction services, allowing researchers to tailor their libraries by choosing specific templates, random peptide insertion sites, variable regions, and desired levels of complexity.
ATHENA II Capsid Library Kits
In partnership with AAVnerGene, we offer premade AAV Capsid Libraries (II) where the CAP gene with random peptides is driven by a CAG/P40 hybrid promoter. The P40 promoter is used during AAV production, while the CAG promoter is used for CAP expression in target cells or tissues. Most premade libraries insert peptides at the VR-VIII and VR-IV regions, which are known for their roles in capsid function.
| SKU | Name | Insert Region | Insert Length | Volume |
|---|---|---|---|---|
| DB005-AAV1 | AAV1 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAV2 | AAV2 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AV3B | AAV3B Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAV5 | AAV5 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAV6 | AAV6 Capsid Libraries |
VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAV7 | AAV7 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAV8 | AAV8 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAV9 | AAV9 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAVrh.10 | AAVrh.10 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
| DB005-AAVrh.74 | AAVrh.74 Capsid Libraries | VR-IV or VR-VIII | NNK7 NNK8 NNK9 |
100ul 200ul 500ul 1000ul |
Notes:
1. Complexity. The complexity was calculated according the numbers of clones after electroporation.
2. AAV library production. The AAV library plasmid was transfected as 200 Copies/cells together with our mini-pHelper-Rep.
3. AAV purification: two rounds of CsCl ultracentrifuge.
4. For the premade AAV capsid library, we only provide AAV virus but not the plasmid.
Contact Us to Order
Custom AAV Capsid Library Design and Production
Custom ATHENA II libraries allow researchers to tailor their AAV capsid libraries by selecting library templates, the length of random peptides, insertion sites, and library complexity. The process involves designing appropriate expression strategies, selecting AAV serotypes, identifying variable regions, and determining peptide insertion or replacement strategies.
AAV Capsid Library Construction Services
We offer generation of random peptide libraries from modified AAV capsid plasmids. The price is dependent on the efficiency and complexity requested. Each library includes amplification of 1mg of endotoxin free plasmid.
|
Library complexity |
Price |
Turnaround Time |
|
1.00E+07 |
$12,800 |
3-4 weeks |
|
1.00E+08 |
$21,800 |
3-4 weeks |
|
1.00E+09 |
$68,000 |
4-5 weeks |
|
Higher |
Please inquire |
|
AAV Capsid Library Production Services
We offer packaging of random peptide libraries from modified AAV capsid plasmid. Our pricing is dependent on quantity and transfection conditions. Pricing increases with lower copies per cell.
|
Scale |
Packaging Conditions |
Price |
Turnaroud Time |
|
2.00E+12 |
1000 copies/cell |
$11,800 |
2-3 weeks |
|
200 copies/cell |
$19,800 |
2-3 weeks |
|
|
100 copies/cell |
$29,800 |
2-3 weeks |
|
|
<50 copies/cell |
Please inquire |
||
|
2.00E+13 |
1000 copies/cell |
$48,800 |
3-4 weeks |
|
200 copies/cell |
$98,000 |
3-4 weeks |
|
|
100 copies/cell |
$148,800 |
3-4 weeks |
|
|
<50 copies/cell |
Please inquire |
||
|
2.00E+14 |
1000 copies/cell |
Please inquire |
|
|
200 copies/cell |
Please inquire |
||
|
100 copies/cell |
Please inquire |
||
|
<50 copies/cell |
Please inquire |
||
AAV Capsid Library Evolution
We can do AAV capsid evolution both in vitro (primary cells and cell lines) and in vivo (mouse, rat, pig and NHP). Please discuss with our technical support for your screening needs.
AAV Services
AAV Vector Design & Cloning
Expert consultation, design, gene synthesis, and cloning of custom AAV vectors tailored to your research needs.
Learn moreAAV Packaging Service
Reliable, affordable, and fully customizable AAV packaging service with diverse serotype options and high titer.
Learn moreAAV QC & Characterization
Comprehensive quality control assays including qPCR, SDS-PAGE, mass photometry, and next-generation sequencing.
Learn moreOther Viruses at Biohippo
Neural Circuit Tracing
Biohippo offers a variety of viral vectors, including RV, HSV, PRV, and VSV, to replace conventional tracers with the highly efficient, more specific, and less invasive viral method.
Learn More
Lentivirus
Biohippo offers TF reporter lentivirus, immunotherapy lentivirus, validated shRNA, cDNA, and miRNA lentiviruses to meet diverse research needs. We also offer customized service at affodable price.
Learn More