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Home / Assay Kits

Viral (Flavivirus) RNA-dependent RNA Polymerase Assay Kit

SKU : BHE16300029

This product includes 350 ul of 10 x Buffer, 350 ul of 10 x MnCl2, 33 ul of 100 x Template, 33 ul of 100 x NTPs (50 mM ATP and 50 mM GTP) and 330 ul of 10 x fluorescence dye. It is for 100 assays of virus RNA polymerase in a 384-well plate format. The assay This product includes all reagents except the enzyme.

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Mfr.No.	VRT100K, VRT500K
Description	The Viral RNA-dependent RNA Polymerase Assay is developed using a RNA polymerase in the Flaviviridae, a family of positive, single-stranded, enveloped RNA viruses. The assay is based on measurement of the RNA molecules synthesized by the RNA polymerase using RNA as a template in the presence of NTPs. The assay can be performed in a 384-well or 96-well plate format for tests of the enzyme activities of RNA polymerases in the Flaviviridae family and high throughput screening of inhibitors. The Viral RNA-dependent RNA Polymerase Assay Kit – 100 (Catalog No.VRT100K) includes 350 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x NTPs (50 mM ATP and 50 mM GTP) and 330 µl of 10 x fluorescence dye. It is for 100 assays of virus RNA polymerase in a 384-well plate format. The assay kit includes all reagents except the enzyme.
Drug target assays	Antiviral drug targets

Reference	Shu Yang et al, Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry, Cell Discovery, Volume 4, Article number: 31, (05 June 2018).

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl. 1.Reaction: The total volume of each reaction mixture is 30 µl including 26.1 µl of H2O, 3 µl of 10 x Buffer, 0.3 µl of 100 x template, 0.3 µl of 100 x RNA polymerase and 0.3 µl of 100 x NTPs. Incubate the reaction mixture at 37°C for 60 min. 2.Detection: Dilute the 10x fluorescence dye with water to make the 1 x dye. Mix 30 µl of the 1 x dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm in 5 min.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300029-1	VRT100K	enzyme not included	100 assays	$365.40		Add to Cart
BHE16300029-2	VRT500K	enzyme not included	500 assays	$1150.18		Add to Cart

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