Ubiquitin Interact Detection Kit

Mfr.No.	SKT-132-20
Alternative Names	Polyubiquitin B Detection Kit, RPS27A Detection Kit, UBA52 Detection Kit, UBB Detection Kit, UBC Detection Kit, ubiquitin B Detection Kit
Categories	Detection Kits
Description	Capture and detection of ubiquitin binding proteins
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	300
Reactivity	Species independent
Scientific Background	The covalent attachment of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events including cell cycle, differentiation, immune responses, DNA repair, chromatin structure, transcription, signal transduction, endocytosis, apoptosis and degradation by the proteasome, autophagy and lysosome systems. As such ubiquitin signalling and the processes it mediates are essential for the normal functioning of cells and its dysfunction has been implicated in wide range of diseases including cancer, neurodegeneration, cardiovascular and metabolic disorders (1,2). The type of ubiquitin modification, (monoubiquitin, multiubiquitin, polyubiquitin), substrate protein lysine residue(s) modified and, in the case of polyubiquitination, the chain length and lysine linkage type control the function and fate of ubiquitinated proteins. In addition all ubiquitin mediated pathways also utilise speciﬁc ubiquitin receptors to facilitate their regulation (3,4). Ubiquitination is achieved through three enzymatic steps. In an ATP-dependent process, the ubiquitin E1 activating enzyme catalyses the formation of a reactive thioester bond with ubiquitin, followed by its subsequent transfer to the active site cysteine of a ubiquitin E2 carrier protein. The selectivity of the ubiquitin cascade for a particular substrate protein relies on the interaction between the E2 conjugating enzyme (of which a cell contains relatively few) and an ubiquitin E3 ligase , of which over 600 have been identified to date. The specific E2-E3 pair required for ubiquitination of a particular substrate protein in vivo may also control the type, point and length / linkage (polyubiquitin) of the ubiquitin modification (5).
Size	800 µl ; 20 tubes ; 20 tubes
Adr Code	Non-hazardous
Assay Overview	The Ubiquitin Interact Detection kit facilitates the selective capture and detection of ubiquitin binding and associated proteins from cell lysates and tissue extracts. The kit utilises a high capacity, high specificity ubiquitin matrix together with an easy-to-use purification system for efficient isolation of ubiquitin binding proteins with minimal non-specific binding. Allows purification of mono- and poly-ubiquitin binding proteins, independent of chain linkage or length. Analysis by Weste blotting or proteomic methods enables identification and assessment of captured proteins. Kit contains sufficient ubiquitin interact matrix to perform up to 20 binding assays.
Assay Type	Binding Matrix/Quantitative WB (Weste Blot)
Assay Type Abbreviation	Purification
Component Number	SKC-132A ; SKC-132B ; SKC-132C
Country Of Origin	Canada
Incubation Time	1 hour
Item	StressXpress® ubiquitin interact matrix (50% slurry) ; StressXpress® columns ; StressXpress® collection tubes
Number Of Samples	20 samples
Platform	Spin Column
Species Reactivity Abbreviations	ALL
Species Reactivity Medium	Species Independent
Trademark	StressXpress®

Target	Ubiquitin Interact
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature	Blue Ice
Storage Temperature	4ºC
Stock Product	Y
Utility	Detection kit used to capture, detect, identify and characterise isolated ubiquitin binding proteins by Weste blotting or proteomic analysis in samples.

Reference	1. Fulda, S., Rajalingam, K. & Dikic, I. EMBO molecular medicine 4, 545–56 (2012). 2. Shaid, S., Brandts, C. H., Serve, H. & Dikic, I. Cell death and differentiation 20, 21–30 (2013). 3. Husnjak, K. & Dikic, I. Annual review of biochemistry 81, 291–322 (2012). 4. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203–229 (2012). 5. Spasser, L. & Brik, A. Angewandte Chemie (Inteational ed. in English) 51, 6840–62 (2012).

Other Resources 1	Kit Booklet
Other Resources 2	MSDS

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BHE11900002	SKT-132-20	20 assays	$403		Add to Cart

Ubiquitin Interact Detection Kit

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