High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.Fast and convenient: homogeneous "mix-and-measure" assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.Robust and amenable to HTS: Z factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
Intended Use
Luminescence
Detection Principle
2 fg luciferase
Shelf Life
12 months
Specifity
Bright bioluminescent reagent system for rapid quantitation of luciferase reporter gene expression in transfected cells and high-throughput drug screens.
Reference
Cohen ME, et al (2011). Antibody against extracellular vaccinia virus (EV) protects mice through complement and Fc receptors. PLoS One. 6(6):e20597. Assay: Reporter gene in monkey cell line.
Mayer-Wagner S et al (2011). Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells. Bioelectromagnetics. 32(4):283-90. Assay: Luciferase reporter gene in monkey vero cells.
Tannous BA et al (2011). Gaussia Luciferase Variant For High-Throughput Screening. WO2011002924. Assay: Reporter gene in mice.
Kulkai AA, et al (2010). Glycan encapsulated gold nanoparticles selectively inhibit shiga toxins 1 and 2. Bioconjug Chem. 21(8):1486-93. Assay: Reporter gene in monkey cell line.
Labuzek K et al (2010). Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures. Naunyn Schmiedebergs Arch Pharmacol. 381(1):41-57. Assay: Luciferase reporter gene in human cell line.
Labuzek K et al (2010). Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures. Pharmacol Rep. 62(5):827-48. Assay: Luciferase reporter gene in human cell line.
McGannon CM, et al (2010). Different classes of antibiotics differentially influence shiga toxin production. Antimicrob Agents Chemother. 54(9):3790-8. Assay: Reporter gene in human cell line.
Roupelieva M, et al (2010). Kaposi's sarcoma-associated herpesvirus Lana-1 is a major activator of the serum response element and mitogen-activated protein kinase pathways via interactions with the Mediator complex. J Gen Virol. 91(5):1138-49. Assay: Reporter gene in human cell line.
Zaliauskiene L, et al (2010). Efficient gene transfection using novel cationic polymers poly(hydroxyalkylene imines). Bioconjug Chem. 21(9):1602-11. Assay: Reporter gene in mice.
Tannous, BA. et al (2009). Secreted Luciferase For Ex Vivo Monitoring Of In Vivo Precesses. US2009235370. Assay: Reporter gene in mice.
Gentry, M. et al. (2007). Role of Primary human Alveolar Epithelial Cells in Host Defense against Francisella tularensis Infection. Infection and Immunity 75(8): 3969-3978. Assay: Reporter gene in human cell line.
Michael, K. et al. (2007). Microplate Orbital Mixing Improves High-Throughput Cell-Based Reporter Assay Readout. J Biomol Screen 12(1):140-144. Assay: Reporter gene in mouse 3T3 cells.
Saenz JB,et al (2007). Identification and Characterization of Small Molecules That Inhibit Intracellular Toxin Transport. Infection and Immunity 75(9): 4552-4561. Assay: Reporter gene in monkey cell line.
Zhao, L. and Haslam, D.B. (2005). A quantitative and highly sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis. J Med Microbiol 54:1023-1030. Assay: Reporter gene in human cell line.
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