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Home / Assay Kits

SARS-Cov-2 RNA polymerase assay kit

SKU : BHE16300026

This product includes 300 ul of 10 x Buffer, 50 ul of 50 x RNA template, 60 ul of 50 x mM NTPs (ATP, UTP, GTP, CTP), 700 ul of 10 x fluorescence dye and 50 ul of 50 x SARS-CoV-2 RdRp. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format.

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Mfr.No.	S2RPA100KE, S2RPA100K, S2RPA020KE
Description	The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is a key drug target for this deadly virus. The SARS-CoV-2 RdRP assay is based on detection of the RNA molecules synthesized by the RNA polymerase in the presence of an RNA template and NTPs. The assay can be performed in a 384-well or 96-well plate format for tests of the enzyme activities of the virus RNA polymerase (SARS-CoV-2 RdRp) and high throughput screening of inhibitors. The SARS-CoV-2 RNA polymerase assay kit plus-100 (Catalog No.S2RPA100KE) includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x mM NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x fluorescence dye and 50 µl of 50 x SARS-CoV-2 RdRp. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format.
Drug target assays	Antiviral drug targets

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 25 µl and the final assay volume is 90 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 50 µl and the final assay volume is 180 µl. 1. Reaction: The total volume of each reaction mixture is 25 µl including 21 µl of H2O, 2.5 µl of 10 x Buffer, 0.5 µl of 50 x template, 0.5 µl of 50 x RNA polymerase and 0.5 µl of 50 x NTPs. Incubate the reaction mixture at 34°C for 2 hours. 2. Detection: Dilute the 10x fluorescence dye with water to make the 1 x dye. Mix 65 µl of the 1 x dye with 25 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm in 10 min.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300026-1	S2RPA100KE	enzyme included	100 assays	$1856		Add to Cart
BHE16300026-2	S2RPA100K	enzyme not included	100 assays	$695		Add to Cart
BHE16300026-3	S2RPA020KE	enzyme included	20 assays	$495		Add to Cart

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