Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.
Intended Use
FL340/450nm
Detection Principle
100 ng/mL
Shelf Life
12 months
Specifity
For quantitative determination of DNA.
Reference
Mayer-Wagner S, et al (2011). Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells. Bioelectromagnetics. 32(4):283-90. Assay: DNA in human stem cell.
Waheed MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology 2011 Jul 13. Assay: DNA in human cell line.
Labuzek K, et al (2010). Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures. Pharmacol Rep. 62(5):827-48. Assay: DNA in rat microglial cells.
Labuzek K, et al (2010). Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures. Naunyn Schmiedebergs Arch Pharmacol. 381(1):41-57. Assay: DNA in rat primary glial culture.
Mayer-Wagner S et al. (2010). Membrane-based cultures generate scaffold-free neocartilage in vitro: influence of growth factors. Tissue Eng Part A. 16(2):513-21. Assay: DNA in human cell.
Bhavnani B.R. et al. (2008). Structure activity relationships and differential interactions and functional activity of various equine estrogens mediated via estrogen receptors (ERs) ERalpha and ERbeta. Endocrinology. 149(10):4857-70. Assay: DNA in human cell line.
Lai, M et al (2007). Development of an In-Vitro Screening Method to Determine Enzymatic Release rates from a Homologous Series of Novel SN-38 Conjugates. Sonus Pharmaceuticals. 2007 AAPS Annual Meeting & Exposition. Assay: DNA in human cells, stem cells.
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