Safe. Non-radioactive assay.Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD670nm
Detection Principle
0.003 U/L
Shelf Life
6 months
Specifity
For quantitative determination of urease activity and evaluation/screen for urease inhibitors.
Reference
Joerger RD, et al (2020) Effect of sodium bisulfate amendments on bacterial populations in broiler litter. Poultry Science.;99(11):5560-5571. Assay: Urease in chicken litter.
Wei, T., et al (2020). Chinese yellow rice wine processing with reduced ethyl carbamate formation by deleting transcriptional regulator dal80p in saccharomyces cerevisiae. Molecules (Basel, Switzerland), 25(16). Assay: Urease in yeast cells.
Liu, Q. W., Liu, Q. Y., Li, J. Y., Wei, L., Ren, K. K., Zhang, X. C. & Xin, H. B. (2018). Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure. Stem cell research & therapy, 9(1), 321. Assay: Urease in human urine.
Wu, D (2015). Constitutive expression of the DUR1, 2 gene in an industrial yeast strain to minimize ethyl carbamate production during Chinese rice wine fermentation. FEMS Microbiology Letters 363(1): fnv214. Assay: Urease.
Zhang, T. et al (2015). Development of an Agrobacterium-mediated transformation system for the cold-adapted fungi Pseudogymnoascus destructans and P. pannorum. Fungal Genetics and Biology 81: 73-81. Assay: urease in fungus.
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