| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, urine, food, and environment |
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Overview
For quantitative determination of sulfate ion and evaluation of drug effects on sulfate metabolism. The assay uses OD600nm for signal readout. Compatible sample input includes Serum, urine, food, and environment. Typical stated assay timing is 5 min.
Key elements and design rationale
- Readout format: OD600nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, urine, food, and environment, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 20 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Detection range 0.02 mM (0.19 mg/dL) to 2 mM (19.2 mg/dL) sulfate in a 96-well plate assay.
Additional feature notes highlight Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 5 min. Available format information for this listing includes 200 Tests.
Biological background
This product is centered on measurement of sulfate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
INORGANIC SULFATE is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics, steroids, neurotransmitters, and bile acids. Sulfate is needed for the biosynthesis of glycosaminoglycans, cerebroside sulfate, and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human-inherited osteochon-drodysplasia disorders. In mammals, sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules, and only 5-20% of the filtered load is excreted into the urine. Simple, direct, and automation-ready procedures for quantitative determination of inorganic sulfate find wide applications in research and drug discovery. BioAssay Systems sulfate assay kit is designed to measure sulfate concentration in biological fluids such as serum and urine. The improved method utilizes the quantitative formation of insoluble barium sulfate in polyethylene glycol. The turbidity measured between 540 and 610nm is proportional to the sulfate level in the sample.
Detection method
Colorimetric (OD 600 nm).
Detection limit and analytical sensitivity
Reported detection limit: 20 µM.
Procedures and timing
Stated procedure or timing information: 5 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify sulfate in serum, urine, food, and environment by OD600 nm readout.
- Compare treatment or phenotype groups using matched serum, urine, food, and environment handling.
- Monitor time-course or pre/post changes in serum, urine, food, and environment across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What is the mechanism of this kit?
This assay is based on barium in the kit forming barium sulfate with sulfate in a sample, which is insoluble. This insoluble compound is measured turbidimetrically.
What samples are compatible with this kit?
This sample has been tested with rat, human, and fetal bovine serum. It has also been tested with human urine. For other samples, we would recommend looking at the “Product Citations” tab to check whether the kit has been used by researchers for your particular sample. Additionally, our
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Charged Glycan Residues Critically Contribute to the Adsorption and Lubricity of Mucins
Marczynski, M et al (2020). Charged Glycan Residues Critically Contribute to the Adsorption and Lubricity of Mucins. Colloids and surfaces. B, Biointerfaces, 187, 110614. Assay: sulfate in bovine mucin.
SdsA1, a Secreted Sulfatase, Contributes to the in Vivo Virulence of Pseudomonas Aeruginosa in Mice
Kida, Y., Yamamoto, T., & Kuwano, K. (2020). SdsA1, a Secreted Sulfatase, Contributes to the in Vivo Virulence of Pseudomonas Aeruginosa in Mice. Microbiology and immunology, 64(4), 280-295. Assay: sulfate in P. aeruginosa culture supernatant filtrate.
Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of rats with Streptozotocin-Induced Diabetes
Sedlak, L., Wojnar, W., Zych, M., Wygledowska-Promienska, D., Mrukwa-Kominek, E., & Kaczmarczyk-Sedlak, I. (2018). Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of rats with Streptozotocin-Induced Diabetes. Nutrients, 10(10), 1423. Assay: Sulfate in rat blood.
Sex-specific effects of serum sulfate level and SLC13A1 nonsense variants on DHEA homeostasis
Tise, C. G., Anforth, L. E., Zhou, A. E., Perry, J. A., McArdle, P. F., Streeten, E. A. & Yerges-Armstrong, L. M. (2017). Sex-specific effects of serum sulfate level and SLC13A1 nonsense variants on DHEA homeostasis. Molecular genetics and metabolism reports, 10, 84-91. Assay: Sulfate in human serum.
A Macro View of a Micronutrient: Revealing the Genetics and Elucidating the Impact of Serum Sulfate on human Health in the Old Order Amish (Doctoral dissertation)
Perry, C. G. (2016). A Macro View of a Micronutrient: Revealing the Genetics and Elucidating the Impact of Serum Sulfate on human Health in the Old Order Amish (Doctoral dissertation). Assay: Sulfate in human serum.
Differentially Expressed Genes in Bordetella pertussis Strains Belonging to a Lineage Which Recently Spread Globally
de Gouw, D., Hermans, P. W., Bootsma, H. J., Zomer, A., Heuvelman, K., Diavatopoulos, D. A., & Mooi, F. R. (2014). Differentially Expressed Genes in Bordetella pertussis Strains Belonging to a Lineage Which Recently Spread Globally. PloS one 9(1): e84523. Assay: Sulfate in B. pertussis cell culture medium.
Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments
Bose, A., Rogers, D. R., Adams, M. M., Joye, S. B., & Girguis, P. R. (2013). Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments. Frontiers in microbiology, 4. Assay: Sulfate in garden banks mud volcano site sediment.
Partial deletion of the sulfate transporter SLC13A1 is associated with an osteochondrodysplasia in the Miniature Poodle breed
Neff, M. W., Beck, J. S., Koeman, J. M., Boguslawski, E., Kefene, L., Borgman, A., & Ruhe, A. L. (2012). Partial deletion of the sulfate transporter SLC13A1 is associated with an osteochondrodysplasia in the Miniature Poodle breed. PloS one 7(12): e51917. Assay: Sulfate in miniature poodle blood, urine.
High-throughput simultaneous analysis of RNA, protein, and lipid biomarkers in heterogeneous tissue samples
Reiser, V et al (2011). High-throughput simultaneous analysis of RNA, protein, and lipid biomarkers in heterogeneous tissue samples. Clinical chemistry 57(11): 1545-1555. Assay: Sulfate in human protein extract from plaque.
Structural characterisation and immunomodulatory property of an acidic polysaccharide from mycelial culture of Cordyceps sinensis fungus Cs-HK1
Wang, ZM et al (2011). Structural characterisation and immunomodulatory property of an acidic polysaccharide from mycelial culture of Cordyceps sinensis fungus Cs-HK1. Food Chemistry 125(2):637-43. Assay: Sulfate in fungi.
Highly sulfated dermatan sulfate from the skin of the ray Raja montagui: anticoagulant activity and mechanism of action
Ben Mansour, M., et al. (2010). Highly sulfated dermatan sulfate from the skin of the ray Raja montagui: anticoagulant activity and mechanism of action. Comp Biochem Physiol B Biochem Mol Biol 156(3):206-15. Assay: Sulfate in fish.
Deletion of the pH sensor GPR4 decreases renal acid excretion
Sun X et al (2010). Deletion of the pH sensor GPR4 decreases renal acid excretion. J Am Soc Nephrol. 21(10):1745-55. Assay: Sulfate in mouse urine.
Characterization of a novel dermatan sulfate with high antithrombin activity from ray skin (Raja radula)
Ben Mansour, M., et al. (2009). Characterization of a novel dermatan sulfate with high antithrombin activity from ray skin (Raja radula). Thromb Res 123(6):887-94. Assay: Sulfate in fish.
Polysaccharides from the skin of the ray Raja radula
Ben Mansour, M., et al. (2009). Polysaccharides from the skin of the ray Raja radula. Partial characterization and anticoagulant activity. Thromb Res 123(4):671-8. Assay: Sulfate in fish.
Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis
Majdoub, H., et al. (2009). Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis. Biochim Biophys Acta 1790(10):1377-81. Assay: Sulfate in Arthrospira platensis.
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