Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 uM (7 ng/mL) to 30 uM (1,020 ng/mL) H2O2 in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Intended Use
OD585nm
Detection Principle
0.2 uM
Shelf Life
12 months
Specifity
For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism.
Reference
Adhikari, B., et al (2020). Cold plasma seed priming modulates growth, redox homeostasis and stress response by inducing reactive species in tomato (Solanum lycopersicum). Free Radical Biology & Medicine, 156, 57-69. Assay: Peroxide in tomato leaves.
Liu, Z.,et al (2021). ROS-responsive and multifunctional anti-Alzheimer prodrugs: Tacrine-ibuprofen hybrids via a phenyl boronate linker. European Journal of Medicinal Chemistry, 212, 112997. Assay: Peroxide in antioxidant solution.
Zhang, Q., Zhang, F., Li, S., Liu, R., Jin, T., Dou, Y. & Zhang, J. (2019). A Multifunctional Nanotherapy for Targeted Treatment of Colon Cancer by Simultaneously Regulating Tumor Microenvironment. THERANOSTICS, 9(13), 3732-3753. Assay: Peroxide in Z-VAD-FMK cells.
Riedemann, I (2017). Insights Into the Mechanisms of Remote ischemic Preconditioning. PhD Dissertation the Kiel University. Assay: Peroxide in human endothelial cells.
Fedeles, B.I., et al. (2011). Chemical genetics analysis of an aniline mustard anticancer agent reveals complex I of the electron transport chain as a target. J Biol Chem 286(39):33910-20. Assay: Peroxide in human cancer cell.
Peng, A., et al. (2011). The green tea polyphenol (-)-epigallocatechin-3-gallate ameliorates experimental immune-mediated glomerulonephritis. Kidney Int. 80(6):601-11. Assay: Peroxide in mouse kidney tissue, urine, serum.
Jawed, H., et al. (2010). N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5. Assay: Peroxide in rat plasma/serum.
Toschi, A., et al. (2010). Phospholipase D-mTOR requirement for the Warburg effect in human cancer cells. Cancer Lett 299(1):72-9. Assay: Peroxide in human cells.
Cunningham, R.L., et al. (2009). Androgens induce dopaminergic neurotoxicity via caspase-3-dependent activation of protein kinase Cdelta. Endocrinology 150(12):5539-48. Assay: Peroxide in rat dopaminergic cell line n27.
Deshmane, S.L., et al. (2009). Activation of the oxidative stress pathway by HIV-1 Vpr leads to induction of hypoxia-inducible factor 1alpha expression. J Biol Chem 284(17):11364-73. Assay: Peroxide in human microglia cell line.
Minamishima, S., et al. (2009). Hydrogen sulfide improves survival after cardiac arrest and cardiopulmonary resuscitation via a nitric oxide synthase 3-dependent mechanism in mice. Circulation 120(10):888-96. Assay: Peroxide in mouse serum.
Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxide in plant rice anther.
Schnabl, K.L., et al. (2009). Gangliosides protect bowel in an infant model of necrotizing enterocolitis by suppressing proinflammatory signals. J Pediatr Gastroenterol Nutr 49(4):382-92. Assay: Peroxide in human intestinal tissue.
Shin, D., et al. (2009). Dicer ablation in oligodendrocytes provokes neuronal impairment in mice. Ann Neurol 66(6):843-57. Assay: Peroxide in mouse brain tissue.
Zhang, H., et al. (2009). Resveratrol improves endothelial function: role of TNF{alpha} and vascular oxidative stress. Arterioscler Thromb Vasc Biol 29(8):1164-71. Assay: Peroxide in mouse serum.
Lens, M., et al. (2008). Antioxidative capacity of C(60) (buckminsterfullerene) and newly synthesized fulleropyrrolidine derivatives encapsulated in liposomes. Biotechnol Appl Biochem 51(Pt 3):135-40. Assay: Peroxide in liposomes.
Zhang, R., et al. (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis 13(12):1465-78. Assay: Peroxide in human pancreatic cancer cells.
Hashimoto, T., et al. (2007). Lactate sensitive transcription factor network in L6 cells: activation of MCT1 and mitochondrial biogenesis. FASEB J 21(10):2602-12. Assay: Peroxide in rat L6 cell lysate.
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