Fast and sensitive. Use as little as 10 uL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD570nm,or FL530/585nm
Detection Principle
OD, FL: 4, 0.8 U/L
Shelf Life
12 months
Specifity
For quantitative determination of peroxidase activity.
Reference
Wada, A., et al. (2021). Protective effect of luminal uric acid against indomethacin-induced enteropathy: Role of antioxidant effect and gut microbiota. Digestive Diseases and Sciences. Assay: Peroxidase in mouse ileal mucosa.
Singh, B., et al. (2020). Oxidative stress associated metabolic adaptations regulate radioresistance in human lung cancer cells. Joual of Photochemistry and Photobiology. B, Biology 213. Assay: Peroxidase in human lung cancer cells .
Jeon, G., et al. (2021). Melanin-decolorizing activity of antioxidant enzymes, glutathione peroxidase, thiol peroxidase, and catalase. Molecular Biotechnology 63(2): 150-155. Assay: Peroxidase in lysosomal fraction .
Sharma, S., et al. (2020). Heat-induced endoplasmic reticulum stress in soleus and gastrocnemius muscles and differential response to UPR pathway in rats. Cell Stress & Chaperones. Assay: Peroxidase in rat muscle .
Ismail, I. B., Al-Busadah, K. A., & El-Bahr, S. M. (2013). Oxidative stress biomarkers and biochemical profile in broilers chicken fed zinc bacitracin and ascorbic acid under hot climate. Am J Biochem Mol Biol, 3, 202-214. Assay: Peroxidase in broiler chicks liver cells.
Hokari, R., et al. (2011). Increased expression of lipocalin-type-prostaglandin D synthase in ulcerative colitis and exacerbating role in murine colitis. Am J Physiol Gastrointest Liver Physiol 300(3):G401-8. Assay: Peroxidase in mouse.
Jawed H et al (2010) N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5 Assay: Peroxidase in rat serum.
Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Joual of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther.
Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell.
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