Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 50 U/L for a 30 minute reaction at 37°C.High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
OD405nm
Detection Principle
0.2 U/L
Shelf Life
12 months
Specifity
?-N-Acetylglucosaminidase activity determination in biological samples.
Reference
Zager, R. A., et al. (2021). Iron sucrose ('RBT-3 ') activates the hepatic and renal HAMP1 gene, evoking renal hepcidin loading and resistance to cisplatin nephrotoxicity. Nephrology, Dialysis, Transplantation: Official Publication of the European Dialysis and Transplant Association - European Renal Association 36(3): 465-474. Assay: ?-N-Acetylglucosaminidase in mouse plasma.
Johnson, A. C., et al. (2019). Parenterial iron sucrose-induced renal preconditioning: Differential ferritin heavy and light chain expression in plasma, urine, and internal organs. American Journal of Physiology. Renal Physiology 317(6): F1563-F1571. Assay: ?-N-Acetylglucosaminidase in human urine.
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