Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay.Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day.
Intended Use
OD412nm
Detection Principle
40 U/L
Shelf Life
12 months
Specifity
For quantitative determination of lipase activity.
Reference
Pitino, MA et al (2019). Is frozen human milk that is refused by mother?s own infant suitable for human milk bank donation? Breastfeeding Medicine: The Official Journal of the Academy of Breastfeeding Medicine, 14(4), 271-275. Assay: Lipase in human milk.
Imanikia, S et al (2019). Xbp-1 remodels lipid metabolism to extend longevity. Cell Reports, 28(3), 581-589.e4. Assay: Lipase in nematode lysates.
Panee, J et al (2020). Chronic marijuana use moderates the correlations of serum cholesterol with systemic mitochondrial function and fluid cognition. Mitochondrion, 52, 135-143. Assay: Lipase in human serum.
Li, L et al (2020). Effects of protocatechuic acid on ameliorating lipid profiles and cardio-protection against coronary artery disease in high fat and fructose diet fed in rats. Journal of Veterinary Medical Science, 82(9), 1387-1394. Assay: Lipase in rat serum.
Fassbinder-Orth, C. A., Killpack, T. L., Goto, D. S., Rainwater, E. L., & Shearn-Bochsler, V. I. (2018). High costs of infection: Alphavirus infection reduces digestive function and bone and feather growth in nestling house sparrows (Passer domesticus). PloS one, 13(4), e0195467. Assay: Lipase in sparrow liver.
Ranawade, A., Mallick, A., & Gupta, B. P. (2018). PRY-1/Axin signaling regulates lipid metabolism in Caenorhabditis elegans. PloS one, 13(11), e0206540. Assay: Lipase in worm tissue.
Srihardyastutie, A., & Soeatmadji, D. W. (2018). The Proteomic Analysis of Pancreatic Exocrine Insufficiency Protein Marker in Type 2 Diabetes Mellitus Patients. In IOP Conference Series: Materials Science and Engineering 299(1) p 012021. Assay: Lipase in human blood.
Li, Y., Nguyen, D. N., de Waard, M., Christensen, L., Zhou, P., Jiang, P. & Dalsgaard, T. K. (2017). Pasteurization procedures for donor human milk affect body growth, intestinal structure, and resistance against bacterial infections in preterm pigs. The Journal of nutrition, 147(6), 1121-1130. Assay: Lipase in human milk.
Shen, A., Kim, H. J., Oh, G. S., Lee, S. B., Lee, S. H., Pandit, A. & Cho, E. Y. (2017). NAD+ augmentation ameliorates acute pancreatitis through regulation of inflammasome signalling. Scientific reports, 7(1), 3006. Assay: Lipase in mice serum.
Flanagan, J. L., Khandekar, N., Zhu, H., Watanabe, K., Markoulli, M., Flanagan, J. T., & Papas, E. (2016). Glycerol monolaurate inhibits lipase production by clinical ocular isolates without affecting bacterial cell viability. Investigative ophthalmology & visual science, 57(2), 544-550. Assay: Lipase in bacteria cells.
Molinari, C.E., et al. (2011). The effect of storage at 25 deg C on proteins in human milk. International Dairy Journal 21(4):286-293. Assay: Lipase in human milk.
Tonsberg, H., et al. (2011). Effect of bile on the oral absorption of halofantrine in polyethylene glycol 400 and polysorbate 80 formulations dosed to bile duct cannulated rats. J Pharm Pharmacol 63(6):817-24. Assay: Lipase in rat intestine.
Freitag, T.L., et al. (2010). human risk allele HLA-DRB1*0405 predisposes class II transgenic Ab0 NOD mice to autoimmune pancreatitis. Gastroenterology 139(1):281-91. Assay: Lipase in mouse serum.
Hu, M., et al. (2010). Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). Journal of Experimental Marine Biology and Ecology 395(1-2):112-9. Assay: Lipase in horseshoe crab blood.
Nachnani, J.S., et al. (2010). Biochemical and histological effects of exendin-4 (exenatide) on the rat pancreas. Diabetologia 53(1):153-9. Assay: Lipase in rat serum.
Hasan, F., et al. (2009). Methods for detection and characterization of lipases: A comprehensive review. Biotechnol Adv 27(6):782-98. Assay: Lipase in microbes.
Narbonne, P., Roy, R. (2009). Caenorhabditis elegans dauers need LKB1/AMPK to ration lipid reserves and ensure long-term survival. Nature 457(7226):210-4. Assay: Lipase in C.elegans larvae.
Qatanani, M., et al. (2009). Macrophage-derived human resistin exacerbates adipose tissue inflammation and insulin resistance in mice. J Clin Invest 119(3):531-9. Assay: Lipase in mouse muscle tissue.
Gabbi, C., et al. (2008). Pancreatic exocrine insufficiency in LXRbeta-/- mice is associated with a reduction in aquaporin-1 expression. Proc Natl Acad Sci U S A 105(39):15052-7. Assay: Lipase in mouse serum.
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