Sensitive and accurate. Use as little as 5 uL samples. Linear detection range 0.7 mg/dL (39 uM) to 300 mg/dL (16.6 mM) glucose in 96-well plate.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum and plasma samples.
Intended Use
OD630nm (Chemical)
Detection Principle
0.7 mg/dL (39 uM)
Shelf Life
12 months
Specifity
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
Reference
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Lambert, K., et al. (2021). Grape polyphenols and exercise training have distinct molecular effects on cardiac hypertrophy in a model of obese insulin-resistant rats. The Journal of Nutritional Biochemistry 87: 108522. Assay: Glucose in rat blood.
Zhang, Y., et al. (2020). Edgeworthia gardneri (Wall.) Meisn. water extract ameliorates palmitate induced insulin resistance by regulating IRS1/GSK3?/FoxO1 signaling pathway in human HepG2 hepatocytes. Frontiers in Pharmacology 10: 1666. Assay: Glucose in human hepatocyte.
Wong, S. K., et al. (2019). Leptin, adiponectin and insulin as regulators for energy metabolismin a rat model of metabolic syndrome. Sains Malaysiana 48(12): 2701-2707. Assay: Glucose in rat serum.
Alghadir, A. H., et al. (2021). Moderate aerobic training modulates cytokines and cortisol profiles in older adults with cognitive abilities. Cytokine 138: 155373. Assay: Glucose in human serum.
Ticiani, E., et al. (2020). Biochemical and metabolic profiles in in vivo- and in vitro-derived concepti in cattle. Livestock Science 233: 103947. Assay: Glucose in cattle plasma.
Jeong, S., et al. (2021). High fructose drives the serine synthesis pathway in acute myeloid leukemic cells. Cell Metabolism 33(1): 145-159.e6. Assay: Glucose in leukemic cells.
Zhu, M., et al. (2020). Identification and gene mapping of the starch accumulation and premature leaf senescence mutant ossac4 in rice. Journal of Integrative Agriculture 19(9): 2150-2164. Assay: Glucose in rice plant leaf.
Logarbo, J. R., Bonvillain, C. P. (2020). Thermal stress responses of two sympatric crayfishes in Louisiana, Procambarus clarkii Girard, 1852 and Procambarus zonangulus Hobbs & Hobbs, 1990 (Decapoda: Astacoidea: cambaridae). Journal of Crustacean Biology 40(6): 734-738. Assay: Glucose in crayfish hemolymph.
Allred, S., et al. (2020). Effects of acute environmental and handling exposure on physiology and fillet quality of market-sized Channel Catfish. Aquaculture Research 51(5): 1854-1866. Assay: Glucose in catfish plasma.
NV, M., et al. (2019). The effects of annatto tocotrienol on bodycomposition and serum adiponectin, leptinand glucose level in a rat model of androgendeficiency induced by buserelin. Med & Health 14(2): 168-179. Assay: Glucose in rat serum.
Zhang, Y., et al. (2020). Ultraconserved element uc.333 increases insulin sensitivity by binding to miR-223. Aging 12(8): 6667-6679. Assay: Glucose in human and mouse cells.
Duarte, A. I., et al. (2020). Liraglutide protects against brain amyloid-?1-42 accumulation in female mice with early alzheimer 's disease-like pathology by partially rescuing oxidative/nitrosative stress and inflammation. International Journal of Molecular Sciences 21(5). Assay: Glucose in mouse brain cortices.
Kazemi, M., McBreairty, L., Chizen, D., Pierson, R., Chilibeck, P., & Zello, G. (2018). A Comparison of a Pulse-Based Diet and the Therapeutic Lifestyle Changes Diet in Combination with Exercise and Health Counselling on the Cardio-Metabolic Risk Profile in Women with Polycystic Ovary Syndrome: A Randomized Controlled Trial. Nutrients, 10(10), 1387. Assay: Glucose in human plasma.
Amrithraj, A. I., Kodali, A., Nguyen, L., Teo, A. K. K., Chang, C. W., Karnani, N. & Stunkel, W. (2017). Gestational diabetes alters functions in offspring's umbilical cord cells with implications for cardiovascular health. Endocrinology, 158(7), 2102-2112. Assay: Glucose in human cells.
Chen, X., Xu, H., Wu, N., Liu, X., Qiao, G., Su, S. & Lin, X. (2017). Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. Oncotarget 8(18): 30305-30316. Assay: Glucose in human lung tissue/plasma.
McMurphy, T. B. (2017). Environmental and gene therapy approaches to improve glycemic control and promote healthy aging (Doctoral dissertation, The Ohio State University). Assay: Glucose in mice blood.
Toorie, A. M., Cyr, N. E., Steger, J. S., Beckman, R., Farah, G., & Nillni, E. A. (2016). The nutrient and energy sensor Sirt1 regulates the hypothalamic-pituitary-adrenal (HPA) axis by altering the production of the prohormone convertase 2 (PC2) essential in the maturation of corticotropin-releasing hormone (CRH) from its prohormone in male rats. Journal of Biological Chemistry, 291(11), 5844-5859. Assay: Glucose in Sprague Dewley rats plasma.
Chiu LL, Radisic M (2010). Scaffolds with covalently immobilized VEGF and Angiopoietin-1 for vascularization of engineered tissues. Biomaterials 31(2):226-41. Assay: Glucose in human endothelial cells.
Hedbacker, K et al (2010). Antidiabetic effects of IGFBP2, a leptin-regulated gene. Cell Metab. 11(1):11-22. Assay: Glucose in mouse blood.
Olsen AS, et al (2010). Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound Repair Regen. 18(5):532-42. Assay: Glucose in zebrafish blood.
Vaitheesvaran B, et al (2010). MKR mice have increased dynamic glucose disposal despite metabolic inflexibility, and hepatic and peripheral insulin insensitivity. Diabetologia 53(10):2224-32. Assay: Glucose in mice serum.
Cirrik S, Oner G. (2009). The effect of heavy muscle activity on renal cytoresistance in rats. Ren Fail. 31(8):683-9. Assay: Glucose in rat urine.
Li, L et al (2009). The nuclear orphan receptor COUP-TFII plays an essential role in adipogenesis, glucose homeostasis, and energy metabolism. Cell Metab. 9(1):77-87. Assay: Glucose in mouse plasma.
Sekowska, A et al (2009). Repulsion and metabolic switches in the collective behavior of bacterial colonies. Biophys J. 97(3):688-98. Assay: Glucose in bacteria cell lysate.
Sullivan JP, et al (2008). Convection and hemoglobin-based oxygen carrier enhanced oxygen transport in a hepatic hollow fiber bioreactor. Artif Cells Blood Substit Immobil Biotechnol. 36(4):386-402. Assay: Glucose in bioreactors daily media.
Zheng Y, et al (2007). Evaluation of different biomass materials as feedstock for fermentable sugar production. Appl Biochem Biotechnol. 137-140(1-12):423-35. Assay: Glucose in wood enzyme.
Jatana M, et al (2006). Inhibition of NF-kappaB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia. J Neuroinflammation 3:12. Assay: Glucose in rat plasma.
Stites T, et al (2006). Pyrroloquinoline quinone modulates mitochondrial quantity and function in mice. J Nutr. 136(2):390-6. Assay: Glucose in mice plasma.
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