| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Food and Beverages |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of free amino nitrogen in foods and beverages. The assay uses OD575nm for signal readout. Compatible sample input includes Food and Beverages. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD575nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food and Beverages, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 20 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Fast and sensitive. Linear detection range (5 µL sample): 0.2 to 10 mM for 10 min reaction.
Additional feature notes highlight Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to process thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of free amino nitrogen within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
FREE AMINO NITROGEN (FAN) is the main source of nitrogen necessary for yeast growth and proper fermentation. Fermentation of beer and wine is processed by yeast, which synthesize proteins using available amino acids. When making beer and wine, free amino nitrogen is extracted from amino acids during the formation of the wort or must.BioAssay Systems’ Free Amino Nitrogen assay measures alpha amino acids, ammonia, and end group amino nitrogens. The ninhydrin based reaction is a superior method for determining only alpha amino acids and ammonia compared to the traditional Kjeldahl, which measures nitrogen from all sources. Only requiring low sample volumes, the stable ninhydrin reagent provides a simple and accurate method for determining Free Amino Nitrogen concentrations.
Detection method
Colorimetric (OD 575 nm).
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify free amino nitrogen in food and Beverages by OD575 nm readout.
- Compare treatment or phenotype groups using matched food and Beverages handling.
- Monitor time-course or pre/post changes in food and Beverages across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How do I store the kit?
This kit is shipped at room temperature. Upon receiving, please store the Reagent A, Reagent B, and Standard in the refrigerator (4°C).
Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommended.
I am not obtaining consistent data, what could be the problem?
This assay is pH dependent; therefore, temperature can affect the results. Be sure to equilibrate all components to room temperature before each assay.
My reader is only accurate to OD 1.0, Can I use this assay on it?
The assay standards reach ODs less than 1.0. If your reader is not accurate to OD 2.0, we suggest diluting samples at least ten-fold in dH2O so they fall within this range and are corrected for dilution factor in calculations.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol.
What samples have you tested?
The kit has been tested on completely fermented light beers, dark beers, beer wort at various stages (post-mash, start of boil, and end of fermentation), and red and white wines.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
