Sensitive and accurate. Linear detection range 7 ug/dL (1.0 uM) to 300 ug/dL (47 uM) copper in 96-well plate assay.Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Intended Use
OD359nm
Detection Principle
7 ug/dL (1.0 uM)
Shelf Life
12 months
Specifity
For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism.
Reference
Hendricks, M. R., et al (2021). Extracellular vesicles promote transkingdom nutrient transfer during viral-bacterial co-infection. Cell Reports, 34(4), 108672. Assay: Copper in bacteria media.
Matte, J. J., & Audet, I. (2020). Mateal perinatal transfer of vitamins and trace elements to piglets. Animal: An Inteational Joual of Animal Bioscience, 14(1), 31-38. Assay: Copper in pig serum.
Braunschmid, V., et al. (2020). A fungal ascorbate oxidase with unexpected laccase activity. Inteational Joual of Molecular Sciences, 21(16). Assay: Copper in yeast cells.
Lai, A., et al. (2019). 72-Hour in vivo evaluation of nitric oxide generating artificial lung gas exchange fibers in sheep. Acta Biomaterialia, 90, 122-131. Assay: Copper in sheep tissue and serum.
Parmar, A., Pascali, G., Lerra, L., Yee, E., Ahmed-Cox, A., Kimpton, K. & Liu, G. J. (2018). In vivo 64Cu CuCl2 PET imaging reveals activity of Dextran-Catechin on tumor copper homeostasis. Theranostics, 8(20), 5645-5659. Assay: Copper in neuroblastoma cell.
Dikicioglu, D., & Oliver, S. G. (2017). Estimating global enzyme abundance levels from cofactor requirements: a model-based analysis of the iron metabolism in yeast. bioRxiv, 229104. Assay: Copper in S. cerevisiae cells.
Yee, E. M., Brandl, M. B., Pasquier, E., Cirillo, G., Kimpton, K., Kavallaris, M. & Vittorio, O. (2017). Dextran-Catechin inhibits angiogenesis by disrupting copper homeostasis in endothelial cells. Scientific reports, 7(1), 7638. Assay: Copper in human cells.
Vittorio, O., Brandl, M., Cirillo, G., Kimpton, K., Hinde, E., Gaus, K. & Haber, M. (2016). Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth. Oncotarget 7(30): 47479-47493. Assay: Copper in human cells.
EL-Deeb, W. M., & El-Bahr, S. M. (2014). Selected Biochemical Indicators of Equine Rhabdomyolysis in Arabian Horses: Acute Phase Proteins and Trace Elements. Joual of Equine Veterinary Science 34(4): 484-488. Assay: Copper in horse serum.
Bartnikas TB (2012) Known and potential roles of transferrin in iron biology. Biometals 25(4):677-86. Assay: Copper in human protein.
Philips N et al (2012) Beneficial regulation of fibrillar collagens, heat shock protein-47, elastin fiber components, transforming growth factor-beta1, vascular endothelial growth factor and oxidative stress effects by copper in dermal fibroblasts. Connect Tissue Res. 53(5):373-8. Assay: Copper in human cell.
Piret JP et al (2012) Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape. Nanotoxicology 6:789-803. Assay: Copper in human cell.
Piret, JP et al (2012). Copper (II) oxide nanoparticles penetrate into HepG2 cells, exert cytotoxicity via oxidative stress and induce pro-inflammatory response. Nanoscale 4(22): 7168-7184. Assay: Copper in human cell culture medium.
Lull ME, et al (2008). Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass. Pediatr Res. 63(6):638-44. Assay: Copper in human plasma.
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