Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well). Convenient. The procedure involves adding a single working reagent and reading after 20 minutes. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.
Intended Use
OD500nm
Detection Principle
8 uM
Shelf Life
12 months
Specifity
Quantitative determination of biotin in food, cosmetic, and biotinylated proteins.