High sensitivity and wide linear range. Use 5 uL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L.Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Intended Use
OD405nm
Detection Principle
2 U/L
Shelf Life
12 months
Specifity
For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate.
Reference
Sul, et al. (2020). Atherogenic diet-induced bone loss is primarily due to increased osteoclastogenesis in mice. The Journal of Nutritional Biochemistry. 79: 108337. Assay: Alkaline Phosphatase in mouse cells.
Harb, S. V., et al. (2020). Hydroxyapatite and ?-TCP modified PMMA-TiO2 and PMMA-ZrO2 coatings for bioactive corrosion protection of Ti6Al4V implants. Materials Science and Engineering: C. 116: 111149. Assay: Alkaline Phosphatase in human osteoblast.
Harper, E., et al. (2020). RANKL treatment of vascular endothelial cells leading to paracrine pro-calcific signaling involves ROS production. Molecular and Cellular Biochemistry. 464(1-2): 111-117. Assay: Alkaline Phosphatase in human cell lysate.
Jeffrey, J. D., et al. (2019). Physiological status of silver carp (Hypophthalmichthys molitrix) in the Illinois River: An assessment of fish at the leading edge of the invasion front. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics. 32, 100614. Assay: Alkaline Phosphatase in silver carp plasma.
Martineau, C., et al. (2020). Preclinical safety and efficacy of 24R,25-dihydroxyvitamin D3 or lactosylceramide treatment to enhance fracture repair. Journal of Orthopaedic Translation. 23: 77-88. Assay: Alkaline Phosphatase in mouse serum.
Moghazy, H., et al. (2020). Protective Effect of Oxytocin Against Bone Loss in a Female Rat Model of Osteoporosis. Reports of Biochemistry & Molecular Biology. 9(2): 147-155. Assay: Alkaline Phosphatase in rat serum.
Niedermair, T., et al. (2020). Absence of ?-calcitonin gene-related peptide modulates bone remodeling properties of murine osteoblasts and osteoclasts in an age-dependent way. Mechanisms of Ageing and Development, 189, 111265. Assay: Alkaline Phosphatase in mouse osteoblast.
Kim, H.-W., Kim, Y.-J. (2021). Fabrication of strontium-substituted hydroxyapatite scaffolds using 3D printing for enhanced bone regeneration. Journal of Materials Science. 56(2): 1673-1684. Assay: Alkaline Phosphatase in mouse osteoblast.
Zhao, Z., et al. (2019). Human periodontal ligament stem cell seeding on calcium phosphate cement scaffold delivering metformin for bone tissue engineering. Journal of Dentistry. 91: 103220. Assay: Alkaline Phosphatase in human cell lysate.
Zhao, Z., et al (2019). Human periodontal ligament stem cells on calcium phosphate scaffold delivering platelet lysate to enhance bone regeneration. RSC Advances, 9(70), 41161-41172. Assay: Alkaline Phosphatase in human cell lysate.
Liu, X., et al. (2020). Injectable electrical conductive and phosphate releasing gel with two-dimensional black phosphorus and carbon nanotubes for bone tissue engineering. ACS Biomaterials Science & Engineering. 6(8): 4653-4665. Assay: Alkaline Phosphatase in human cell lysate.
Chen, H., et al. (2020). An antibacterial and injectable calcium phosphate scaffold delivering human periodontal ligament stem cells for bone tissue engineering. RSC Advances. 10(66): 40157-40170. Assay: Alkaline Phosphatase in human cell lysate.
Niedermair, T., et al. (2020). Impact of the sensory and sympathetic nervous system on fracture healing in ovariectomized mice. International Journal of Molecular Sciences. 21(2): 405. Assay: Alkaline Phosphatase in mouse serum.
Murali, V. P., et al. (2020). Modified electrospun chitosan membranes for controlled release of simvastatin. International Journal of Pharmaceutics. 584: 119438. Assay: Alkaline Phosphatase in mouse cell lysate.
Niedermair, T., et al. (2020). Influence of extracellular vesicles isolated from osteoblasts of patients with cox-arthrosis and/or osteoporosis on metabolism and osteogenic differentiation of bmscs. Frontiers in Bioengineering and Biotechnology. 8: 615520. Assay: Alkaline Phosphatase in human osteoblast.
George, M. N., et al. (2020). Phosphate functionalization and enzymatic calcium mineralization synergistically enhance oligo[poly(Ethylene glycol) fumarate
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