Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD412nm
Detection Principle
10 U/L
Shelf Life
6 months
Specifity
For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.
Reference
Ketenci, S. et al (2020). The neurochemical effects of prazosin treatment on fear circuitry in a rat traumatic stress model. Clinical Psychopharmacology and Neuroscience, 18(2), 219-230. Assay: Acetylcholinesterase in Rat Brain Tissue.
Voorhees, J. R., et al (2019). Occupational-like organophosphate exposure disrupts microglia and accelerates deficits in a rat model of Alzheimer 's disease. NPJ Aging and Mechanisms of Disease, 5, 3. Assay: Acetylcholinesterase in Rat Plasma.
Hafiz, Z. Z., et al (2020). Inhibitory effects of raw-extract centella asiatica (Reca) on acetylcholinesterase, inflammations, and oxidative stress activities via in vitro and in vivo. Molecules (Basel, Switzerland), 25(4). Assay: Acetylcholinesterase in Rat Cells.
Aydin B, Cabadak H, Goren MZ (2018). Investigation of the Roles of Non-neuronal Acetylcholine in Chronic Myeloid Leukemic Cells and their Erythroid or Megakaryocytic Differentiated Lines. Anticancer Agents Med Chem. 18(10):1440-1447. Assay: Acetylcholinesterase in human K562 cells.
Margus, A. (2018). Adaptation to stressful environments: invasion success of the Colorado potato beetle (Leptinotarsa decemlineata). JYU dissertations, (11). Assay: Acetylcholinesterase in beedle larvae.
McCloskey, M et al (2017). The influence of acetylcholinesterase on anxiety- and depression-like behaviors in fluoxetine-treated male mice. BIOS 88(1), 29-38. Assay: Acetylcholinesterase in mice brain cells.
Musa, N. H., Mani, V., Lim, S. M., Vidyadaran, S., Majeed, A. B. A., & Ramasamy, K. (2017). Lactobacilli-fermented cow's milk attenuated lipopolysaccharide-induced neuroinflammation and memory impairment in vitro and in vivo. Journal of Dairy Research, 84(4), 488-495. Assay: Acetylcholinesterase in mice brain cells.
Sorial, M. E., & El Sayed, N. S. E. D. (2017). Protective effect of valproic acid in streptozotocin-induced sporadic Alzheimer's disease mouse model: possible involvement of the cholinergic system. Naunyn-Schmiedeberg's archives of pharmacology, 390(6), 581-593. Assay: Acetylcholinesterase in mice.
Mineur YS, et al. (2013) Cholinergic signaling in the hippocampus regulates social stress resilience and anxiety- and depression-like behavior. Proc Natl Acad Sci U S A 110: 3573-3578. Assay: Acetylcholinesterase in mouse brain nuclei.
Khokhar JY and Tyndale RF (2012). rat brain CYP2B-enzymatic activation of chlorpyrifos to the oxon mediates cholinergic neurotoxicity. Toxicol Sci. 126:325-35. Assay: Acetylcholinesterase in rat serum.
Gupta, A et al (2011). Peripheral insulin-sensitizer drug metformin ameliorates neuronal insulin resistance and Alzheimer's-like changes. Neuropharmacology 60(6):910-20. Assay: Acetylcholinesterase in neuronal cell line.
Ng J, et al (2011). Autonomic remodeling in the left atrium and pulmonary veins in heart failure: creation of a dynamic substrate for atrial fibrillation. Circ Arrhythm Electrophysiol.4(3):388-96. Assay: Acetylcholinesterase in dog explanted atria.
Al-Akwa AA, et al (2009). Free radicals are present in human serum of Catha edulis Forsk (Khat) abusers. J Ethnopharmacol. 125(3):471-3. Assay: Acetylcholinesterase in human serum.