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Home / Assay Kits / Biochemical Kits

Protein and DNA Removal Spin-columns

BHE16300402

This product includes 20 pre-packed spin-columns. Each column is to load 0.2 ml of sample.

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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
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Ask Merchant
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Product Manual Datasheet
Protocols Description PROTOCOLS
1. Spin the pre-packed columns briefly using a bench-top microcentrifuge to set down the resin. Cut off the caps of 1.5 ml-eppendorf tubes and use the tubes as receivers of the spin columns. Remove the column bottom tips and caps. Place the columns into the1.5 ml-eppendorf tubes and spin the columns at 13,000 rpm for 1 min. Discard the solution. Load 200 µl of water and spin the columns at 13,000 rpm for 1 min. Discard the solution. Transfer each column into a clean labeled 1.5-ml eppendorf tube.
2. Load 200 µl of the sample in a buffer containing 100 mM NaCl onto each column and spin the columns at 1000 rpm for 1 min. Then continue to spin the columns at 13,000 rpm for 1 min and save the elute.
 
Note: Step 2 can be repeated if the sample volume is more than 200 µl until the column binding capacity is reached. Inclusion of 100 mM NaCl is not necessary for removal of protein or DNA but helps to minimize binding of small charged molecules on the column.
 
3. Centrifuged the elute at 13,000 rpm for 1 min to remove any insoluble material. Alternatively, the elute can be filtrated with a 0.22 µm to remove any insoluble material.
SKU Supplier No. Size Your price Quantity
BHE16300402 PNR020 20 columns $230.17
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