P. aeruginosa MurD Assay Kit Plus-100

Mfr.No.	MURD100KP
Description	Antibacterial Drug Target. MurD is a D-Glutamic acid-adding enzyme in the pathway for bacterial cell-wall peptidoglycan synthesis. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurD catalyses the addition of D-glutamic acid to UDP-MurNAc-L-Ala, generating UDP-MurNAc-dipeptide. The ligation reaction uses ATP hydrolysis as an energy source forming ADP and inorganic phosphate. The Bacterial MurD Assay is based on measurement of the inorganic phosphate generated from the MurD reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of bacterial MurD in drug discovery research. It may also be used for characterization of bacterial MurD. This product includes the reagents for 100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.035 ml of 100 x D-Glu, 0.035 ml of 100 x ATP , 0.035ml of 100 x P. aeruginosa MurD (2000 nM) and 5 ml of Dye for phosphate detection.
Drug target assays	Antibacterial drug targets

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300056	MURD100KP	100 assays	$613.14		Add to Cart