P. aeruginosa IspD Assay Kit Plus-500

Mfr.No.	ISPD500KP
Description	Antibacterial Drug Target. Methylerythritol phosphate cytidyltransferase (IspD) is one of the enzymes in the non-mevalonate pathway for isoprenoid biosynthesis that present in many pathogenic organisms and plants but absent in mammals. IspD is an attractive target for the development of novel antibiotics and herbicides. This enzyme catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). The Bacterial Methylerythritol Phosphate Cytidyltransferase (IspD) Assay is based on measurement of the pyrophosphate generated from the IspD reaction. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay is in a 384-well or 96-well plate format. It can be used for high throughput screening in drug discovery. This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer,0.170 ml of 100 x MEP, 0.170 ml of 100 x CTP, 0.170 ml of 100 x P. aeruginosa IspD (200 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection.
Drug target assays	Antibacterial drug targets

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300130	ISPD500KP	500 assays	$1863.89		Add to Cart