This kit can be used for quantitative determination of NEFA in serum, plasma, tissue homogenate, cells (or cell supernatant).
Description
Biochemical
Assay Type
Biochemical kit
Detection Instrument
Microplate reader, Biochemistry analyzer
Detection Method
Colorimetric method
Detection Principle
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD), then react with TOOS and a colored substrate will be generated under the catalysis of peroxidase (POD)
Experiment Instruments
Microplate reader, Biochemistry analyzer
Manual
Target
NEFA
Storage
Reagent 1: 4℃
Reagent 2: 4℃
Valid Period
6 months, 6
Manual
Reviews of Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit