Neutrophil Elastase Inhibitor Screening Assay Kit

Mfr.No.

82090

Synonyms

Bone marrow serine protease, Elastase-2, Human leukocyte elastase (HLE), Medullasin, PMN elastase, ELANE, ELA2

Background

Elastase is a serine protease from the chymotrypsin family involved in the degradation of elastin and some viral proteins. Elastase exists as pancreatic and neutrophil elastase, which differ in their specificity. Neutrophil elastase is also known as ELA2 or polymorphonuclear leukocyte elastase and is present in azurophilic granules. Upon neutrophil activation, degranulation occurs and serine proteases, such as elastase, are released into the extracellular space to degrade the matrix proteins of pathogens. Alterations in the levels of elastase, or dysfunction of its endogenous inhibitors, result in alpha-1 antitrypsin deficiency (A1AD), emphysema, cyclic neutropenia, abdominal aortic aneurysms (AAA) and chronic obstructive pulmonary disease (COPD), arthritis and psoriasis. The use of neutrophil elastase inhibitors, such as elafin, and the development of new inhibitors with fewer side effects may benefit patients suffering from elastase-related diseases.

Description

The Neutrophil Elastase Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of elastase for screening and profiling applications. The elastase assay kit comes in a convenient 96-well or 384-well format and contains enough purified elastase, fluorochrome-conjugated substrate, and PR-02 buffer for 100 or 400 reactions. This kit also includes Sivelestat as control inhibitor.

Illustration of the assay principle. PR Substrate 3 is a fluorogenic peptide substrate for neutrophil elastase. In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Proteolysis releases AMC and fluorescence is emitted. The increase in fluorescence is directly proportional to elastase activity.

Materials Required But Not Supplied

Fluorescent microplate reader capable of reading lexcitation = 360 nm; lemission = 460 nm
Adjustable micropipettor and sterile tips

Kit Components

96 reactions

Name	Amount	Storage
Native Human Neutrophil Elastase*	> 1 µg	-80°C
PR Substrate 3	6.25 µl	-80°C
PR-02 Buffer	10 ml	-20°C
10 mM Sivelestat	20 μl	-80°C
96-well black microplate	1	Room Temp

384 reactions

Name	Amount	Storage
Native Human Neutrophil Elastase*	2 x > 1 µg	-80°C
PR Substrate 3	2 x 6.25 µl	-80°C
PR-02 Buffer	2 x 10 ml	-20°C
10 mM Sivelestat	20 μl	-80°C
96-well black microplate	1	Room Temp

Uniprot	P08246
Usage Notes	BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Shipping Temperature	-80°C
Format	Fluorogenic
Storage	This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Reference	Jakimiuk K, et al., 2021 J Enzyme Inhib Med Chem 36(1): 1016-1028.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE18200002-1	82090-1	96 reactions	$520		Add to Cart
BHE18200002--2	82090-2	384 reactions	$1350		Add to Cart

Neutrophil Elastase Inhibitor Screening Assay Kit

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