This kit can be used to detect MPO activity in various Animal plasma, serum, and tissue.
Description
Biochemical
Assay Type
Biochemical kit
Detection Instrument
Spectrophotometry (Visible range)
Detection Method
Colorimetric method
Detection Principle
Myeloperoxidase (MPO) exists in neutrophils with 5% of dry weight for every cell, well-known of its oxidation of H2O2 and oxychloride to HCLO. It will be released to extracellular fluid for incycle in inflammation situation. And MPO also attend reaction with LDL. With its reducibility, MPO react with o-methoxyaniline. Therefore, it can calculate MPO activity, cell number, and the reduced amount of H2O2. by colorimetric analysis at 460 nm.
Detection Range
2.9-393.3U/L
Experiment Instruments
Spectrophotometry (Visible range)
Inter-cv (%)
6.66
Intra-cv (%)
3.9
Manual
Recovery
104
Sensitivity
2.9U/L
Target
MPO
Storage
Reagent 1: 4℃
Reagent 2: 4℃
Reagent 3(A): 4℃
Reagent 3(B): 4℃
Reagent 4: RT
Reagent 5: 4℃
Reagent 6: 4℃
Reagent 7: 4℃
Valid Period
6 months, 6
Manual
Reviews of Myeloperoxidase (MPO) Activity Assay Kit