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Home / Assay Kits / Concentration Measurement Assays

MicroMolar Sulfate Assay Kit

BHE16300288

This product includes 40 ml of Reagent BP. It is for assays using 96-well plates. Cuvettes may also be used for measurements.

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+1 866.986.9598
Email
orders@biohippo.com
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Product Manual Datasheet
Protocols Description ASSAY PROTOCOL
The following assay protocol is based on using a 96-well plate for the measurement. The sample volume is 100 µl and the final assay volume is 300 µl. For 384-well plate assays, the sample volume is 30 µl and the final assay volume is 90 µl. For assays using cuvette, the sample volume is 333 µl and the final assay volume is 999 µl.
 
STANDARD CURVE
1. Sample preparation: Prepare 100 µl of sulfate solutions in the wells of a transparent 96-well plate with a two-fold serial dilution from 5 mM to zero in a 10 mM HEPES, pH 7.4 buffer.
 
2. Detection: Mix 200 µl of Reagent BP with 100 µl of the sulfate solutions for 5 min and read the OD600.
 
3. Data Analysis: Plot the OD600 and the sulfate concentration [Sulfate] to generate the linear standard curve.
 
                                                     OD600 = a [Sulfate] + b
 
Where the OD600 values are from experimental data, the a and b values are from the linear fitting between the OD600 values and the sulfate concentrations.
 
UNKNOWN SAMPLES
Follow the same procedure to measure the OD600 values from the unknown samples. Calculate the sulfate concentrations in the unknown samples using the OD600 values from the unknown samples and the a and b values from the standard curve.
 
                                                   [Sulfate] = (OD600 – b) / a
SKU Supplier No. Size Your price Quantity
BHE16300288 MSA200 200 assays $371.31
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