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Home / Assay Kits

MicroMolar Cysteine Assay kit

BHE16300247

This product includes 400 ul of 100 x Dye R53. It is for measurement of 200 samples using 96-well plates. Cuvettes or 383-well plates may also be used for measurements.

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Tel
+1 866.986.9598
Email
[email protected]
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Reference  Ho M.L et al, Silver nanoprism-based paper as ratiometric sensor for extending biothiol detection in serum. New Journal of Chemistry. Oct 30 (2017).  

Wang LJ et al, Increased serum levels of cysteine in patients with schizophrenia: A potential marker of cognitive function preservation. Schizophrenia Research, Volume 192, Pages 391–397 (2018).  
Product Manual Datasheet
Protocols Description PROTOCOL
The following assay protocol is based on using a 96-well plate for the measurement. The sample volume is 100 µl and the final assay volume is 300 µl. For 384-well plate assays, the sample volume is 30 µl and the final assay volume is 90 µl. For assays using cuvette, the sample volume is 333 µl and the final assay volume is 999 µl.
 
STANDARD CURVE
1. Sample preparation: Freshly prepare 100 µl of cysteine solutions in a 96-well black plate with a two-fold serial dilution from 0.050 mM to zero in water or a 10 mM HEPES, pH 7.4 buffer. For 10 samples, dilute 22 µl of the 100 x R53 dye 100-fold with water to make 2.2 ml of 1 x R53 dye.
 
2. Detection: Mix 200 µl of 1 x dye R53 with 100 µl of the cysteine solutions and immediately read the fluorescence at 535 nm with excitation at 485 nm.
 
Note: Cysteine is quickly oxidized by oxygen in the air or solution. The cysteine solution at low concentrations must be freshly prepared before the measurement. After mixing the dye and the cysteine sample, the fluorescence intensity is read immediately.
 
3. Data Analysis: Plot the fluorescence intensity Fc and the cysteine concentration [Cysteine] to generate the linear standard curve.
 
                                           Fc = a [Cysteine] + b
 
Where the Fc values are from experimental data, the a and b values are from the linear fitting between the Fc values and the cysteine concentrations.
 
UNKNOWN SAMPLES
Follow the same procedure to measure the fluorescence intensity Fc values from the unknown samples. Calculate the cysteine concentrations in the unknown samples using the Fc values from the unknown samples and the a and b values from the standard curve.
 
                                         [Cysteine] = (Fc – b) / a
SKU Supplier No. Size Your price Quantity
BHE16300247 CYS200 200 assays $302.17
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