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Home / Assay Kits / Concentration Measurement Assays

MicroGram Phosphatidylcholine Assay Kit

SKU : BHE16300258

This product includes MPC reagent A, MPC reagent B, 100 x MPC reagent C, MPC reagent D, and a standard solution of L-alpha-phosphatidylcholine (1 mg/ml). The assay kit sufficient for measurement of 200 samples of L-alpha-phosphatidylcholine using 96-well plates (Costar 3915 and Greiner 655076).

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Mfr.No.	MPC200K
Description	The MicroGram Phosphatidylcholine Assay Kit is for measurement of L-αphosphatidylcholine (PC) at concentrations of 0.005% (0.05 mg/ml) or higher. The assay is based on the principle that phosphatidylcholine interacts with the MPC reagent and enhances the fluorescence intensity at 535 nm (excitation at 485 nm). L-α-phosphatidylglycerol (PG) does not interact with the MPC reagent. The assay kit is sufficient for measurement of 200 samples using 96-well plates (Costar 3915 and Greiner 655076). The assay is compatible with most biochemical assay buffers such as HEPES or Tris-HCl buffers. It is compatible with low concentrations of Ethanol and DMSO. It is not compatible with phosphate buffers. If ATP is used for the biochemical reaction, it is recommended to use ATP concentrations below 200 µM. The assay is not compatible with most detergents. The assay kit (Catalog number MPC200K) includes 5 ml of Reagent A, 20 ml of Reagent B, 0.2 ml of 100 x Reagent C and 10 ml Reagent D. It is for 200 assays using 96 well plates.
Concentration measurement	Biomolecules

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on using a 96-well plate for the measurement. The sample volume is 25 µl and the final assay volume is 175 µl. For assays using cuvette, the sample volume is 145 µl and the final assay volume is 1015 µl. STANDARD CURVE 1. Sample preparation: Prepare 25 µl of L-α-phosphatidylcholine (PC) solutions in the wells of a black 96-well plate with a two-fold serial dilution from 1 mg/ml to zero in methanol. For each 10 samples, dilute 0.01 ml of 100 x Reagent C with 0.99 ml of Reagent B to make Reagent BC. 2. Detection: Mix 25 µl of Reagent A with 25 µl of the PC solutions then add 100 µl of Reagent BC. After mixing the solution, add 50 µl of Reagent D and incubate the mixture for 5 min. Read the fluorescence at 535 nm (excitation at 485 nm). 3. Data Analysis: Plot the fluorescence intensity Fc and the PC concentration [PC] to generate the linear standard curve. Fc = a [PC] + b Where the Fc values are from experimental data, the a and b values are from the linear fitting between the Fc values and the PC concentrations. UNKNOWN SAMPLES Follow the same procedure to measure the fluorescence intensity Fc values from the unknown samples. Calculate the PC concentrations in the unknown samples using the Fc values from the unknown samples and the a and b values from the standard curve. [PC] = (Fc – b) / a

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300258	MPC200K	200 assays	$331.38		Add to Cart

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