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Home / Assay Kits

Liposome Drug Encapsulation Assay Kit

BHE16300407

This product includes 30 prepacked spin-columns and 10 ml of elution buffer. It is for measurement of drug encapsulation of 10 liposome samples.

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Tel
+1 866.986.9598
Email
[email protected]
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Product Manual Datasheet
Protocols Description ASSAY PROTOCOL
1. Sample preparation
Remove any high aggregates by filtration of the samples through a 0.22 µm filter.
 
2. Column preparation
(1) Use 3 spin columns for each sample (triplicate measurement). Briefly spin the columns to set down the resin. Remove the caps of 1.5-ml Eppendorf tubes and use them as receiver tubes. Remove the bottom tip and the cap of each spin column and insert the column into a receiving tube.
(2) Spin the columns at 1000 rpm using a benchtop Eppendorf centrifuge for 1 min and discard the elute.
(3) Repeat Step (2).
(4) Add 150 µl of the elution buffer, spin the columns at 1000 rpm for 2 min and discard the elute.
(5) Spin the columns at 1000 rpm for 4 min and change to a clean receiving tube.
 
3. Separation of encapsulated drugs
(1) Load 50 µl of the liposome sample onto each column.
(2) Spin the column at 1000 rpm for 2 min.
(3) Add 150 µl of the elution buffer on the top of the column and spin the column at 1000 rpm for 4 min.
(4) Mix the elute well by pipetting up-and-down a few times. This is the “Encapsulated” sample.
 
4. Analyze the liposomal drugs
(1) Prepare the sample for the total drug concentration by mixing 50 µl of the sample and 150 µl of the elution buffer. This is the “Total” sample.
(2) Measure the light absorbance values of the “Encapsulated” sample (Ae), “Total” sample (At) and the buffer (Ab) 1 .
(3) Calculate the encapsulation yield (%)
        Encapsulation yield (%) = (Ae – Ab) x 100% / (At – Ab)

1Note: If the drug concentration is too high, serial dilution is needed to avoid saturated signal. If it is UV absorbance, all the samples are solubilized and measured in ethanol or methanol.

 
SKU Supplier No. Size Your price Quantity
BHE16300407 LDE10 10 assays $320.18
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