Safe. Non-radioactive assay (cf. chromium release assay).Fast. The whole procedure take 20 min.Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day.
Intended Use
OD500nm
Shelf Life
12 months
Specifity
For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.Â
Reference
Vergis, J et al (2021). Exploring Galleria mellonella larval model to evaluate antibacterial efficacy of Cecropin A (1-7)- Melittin against multi-drug resistant enteroaggregative Escherichia coli. Pathogens and Disease, ftab010. Assay: LDH Cytotoxicity in galleria mellonella.
Zhou, et al (2019). Retrospective analysis of the efficacy of adjuvant CIK cell therapy in epithelial ovarian cancer patients who received postoperative chemotherapy. OncoImmunology, 8(2), e1528411. Assay: LDH Cytotoxicity in human cells.
Flage, B. (2018). Heme-induced acute lung injury by inflammasome activation in vascular endothelium (Doctoral dissertation, University of Pittsburgh). Assay: Cytotoxicity in human tissues.
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