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Lactate dehydrogenase (LDH) Activity Assay Kit

SKU : BHE10308184

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	E-BC-K046-S
Assay Time	70 min
Assay Type	Enzyme ActIVIty
Inter-cv (%)	7.2
Intra-cv (%)	2.2
Sensitivity	4 U/L
Test Principle	Lactate dehydrogenase (LDH) is an oXidoreductase. LDH catalyzes the conversion of lactate to pyruVIc acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. EXtracellular LDH actIVIty is used to detect cell damage or cell death.

Research Area	Enzymes,Glycolysis & Carbohydrates,LIVer Biomarkers

Dilutions	It isrecommended to take 2~3 samples with expected large difference to dopre-experiment before formal experiment and dilute the sample according to theresult of the pre-experiment and the detection range (0.2-55.6King unit/100 mL. Therecommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, Human serum Human urine Rat serum Cellular supernatant 10% Mouse kidney tissue homogenization 30-50, 10% Mouse liver tissue homogenization HePG2 cell 10% Mouse brain tissue homogenization 1. Note:Thediluentisnormalsaline(0.9%NaCl)orPBS(0.01M,pH7.4).
Storage	2-8°C
Valid Period	6 Months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10308184	E-BC-K046-S	100 assays	$150		Add to Cart

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