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Home / Assay Kits / Signalling Pathway Assays

Kras G12D Nucleotide Exchange Assay Kit

SKU : BHE20700003

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Aurora Biolabs

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Mfr.No.	5727-4123NK
Synonyms	Ras, Kras, cRas,
Description	Kras is a member of the RAS protein family, which are a class of small GTPases involved in cell signaling pathways. The Ras signaling pathway plays an important role in cell proliferation and differentiation. Conversion of Kras from the inactive GDP-bound state to the active GTP-bound state triggers the downstream effector and promotes cell growth. RAS genes are frequently mutated in various human tumors. These mutations block the GTPase activity of RAS and lock RAS in the GTP-bound state, resulting in constitutively active signals through the downstream cascades leading to cancer cell proliferation. The Kras (wild type, WT) nucleotide exchange assay is a TR-FRET based assay. The assay kit is designed to detect the GTP binding status of wild type Kras in the presence of SOS1, the most-studied guanine nucleotide exchange factor (GEF) of Kras. The Tag2 Kras in this assay kit is recognized by a Terbium-labeled anti-Tag2 antibody (HTRF donor). If Kras binds to a fluorescence-labeled GTP (HTRF acceptor), the donor and the acceptor will be brought in close proximity. Excitation of Terbium (340 nm) generates fluorescence resonance energy transfer (FRET) to the fluorescence-labeled GTP acceptor, which consequently fluoresces at 665 nm. Thus, GTP binding to Kras can be quantitively measured by calculation of the fluorescent ratio of 665 nm/620 nm.
Function	High throughput screening of compounds that inhibit Kras activation for drug discovery.
Reactivity	Human
Mutation	G12D

Target	KRAS

Shipping	Dry Ice
Format	TR-FRET, time-resolved fluorescence resonance energy transfer
Storage	1 year (-80?C)

Product Manual	Kras G12D Nucleotide Exchange Assay Kit Information

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SKU	Supplier No.	Size	Your price	Quantity
BHE20700003	5727-4123NK	384 reactions	$1574		Add to Cart
BHE20700003	5727-4123NK	4000 reactions	$12590		Add to Cart

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