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Elabscience Biotechnology Co.,Ltd
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Elabscience Biotechnology Co.,Ltd
view supplier detailsCredit card payments now incur a 3% fee.
| Mfr.No. | E-BC-K062-S |
| Background | Hydroxyproline accounts for 13.4% in collagen and very little in elastin, but cannot be found in other proteins. Collagen is mostly distributed in skin, tendon, cartilage, blood vessels, etc. So the amount of hydroxyproline can reflect the collage metabolism of the connective disease. The determination of hydroxyproline in skin is used as a screening index of anti-aging medicines. The excretion of hydroxyproline will increase with age in development stage, then keep stable after non-development. The hydroxyproline in elderly is less than the young. When rats suffer from spontaneous hypertension and secondary hypertension, the content of hydroxyproline will increase in their urine. Surgical trauma will result in an increase of hydroxyproline in the urinary excretion. High lipid, low protein, alcoholic poisoning toxicant, malnutrition, or liver cell degeneration and necrosis will lead to the increase of fiber by inflammation which splits liver lobule, leading to cirrhosis of the liver. When the liver fibrosis, the collagen fibers are the mainly increasing composition in liver, which uniquely have the hydroxyproline. The content of liver hydroxyproline can be converted to the content of the liver collagen, reflecting the degree of liver fibrosis. The decomposition of collagen fiber mainly depends on collagen enzyme and other protease. The hydroxyproline decomposed products will excrete by the urine. The collagen degradation can be reflected by the content of urinary hydroxyproline. By determining the content of hydroxyproline in the tissue and urine, the degree of fibrosis can be judged, and the preventing and treating drugs can also be screened. |
| Description | This kit can measure HYP content in animal tissue, serum (plasma), culture cells, nutrient solution, body fluid and other samples. |
| Description | Biochemical |
| Assay Type | Biochemical kit |
| Detection Instrument | Spectrophotometry (Visible range) |
| Detection Method | Colorimetric method |
| Detection Range | 0.01-20μg/ml |
| Experiment Instruments | Spectrophotometry (Visible range) |
| Inter-cv (%) | 4.65 |
| Intra-cv (%) | 2.2 |
| Manual | 
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| Recovery | 102 |
| Sensitivity | 0.01μg/ml |
| Manual | 
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