Human Topoisomerase II DNA Decatenation Assay Reagent Set (enzyme not included)
BHE16300018
This product includes the reaction buffer, spin columns and loading solution for 100 assays of DNA decatenation reactions. Enzyme and concatenated DNA are not included in the set.
Anticancer Drug Target. Human DNA topoisomerase II alpha converts the concatenated DNA into decatenated DNA. ProFoldin offers the Human DNA topoisomerase II assays in two assay formats: Spin-column-based DNA decatenation assay and 96-well filter platebased DNA decatenation assay. In the spin-column-based DNA decatenation assay, the decatenated DNA is separated from the concatenated DNA by a quick and easy spin-column process. The concatenated DNA stays on the column, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence. In the 96-well filter platebased DNA decatenation assay, the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm). This product includes the reaction buffer, spin columns and loading solution for 100 assays of DNA decatenation reactions. Enzyme and concatenated DNA are not included in the set.
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