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Home / Assay Kits / Drug Target Assays

Human Mitochondrial RNA Polymerase Assay Kit

BHE16300024

This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x NTP mix, 330 ul of 10 x fluorescence dye. Enzyme is not included

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Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
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Product Manual Datasheet
Protocols Description Assay protocol
The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl.
 
1. Reagent preparation:
(1) 10 x DNA: Dilute the 100 x DNA 10-fold with water. Each assay uses 3 µl of 10 x DNA.
(2) 10 x enzyme: Dilute the 50 x Human Mitochondrial RNA Polymerase 5-fold with the 1 x assay buffer. Each assay uses 3 µl of 10 x enzyme.
(3) 10 x NTP mix: Dilute the 50 x NTP mix 5-fold with water. Each assay uses 3 µl of 10 x NTP mix.
(4) 1 x dye: Dilute the 10 x fluorescence dye 10-fold with water. Each assay uses 30 µl of 1 x dye.
 
2. Reaction:
The total volume of each reaction mixture is 30 µl including 18 µl of H2O, 3 µl of 10 x buffer, 3 µl of 10 x DNA template, 3 µl of 10 x enzyme, 3 µl of 10 x NTP mix. Incubate the reaction mixture at 37°C for 2 hours.
 
3. Detection:
Mix 30 µl of 1 x dye with 30 µl of the assay reaction mixture for 5 min and read the fluorescence at 535 nm (excitation at 485 nm).
Combination SKU Supplier No. Specifications Size Your price Quantity
BHE16300024-1 MRPA100K enzyme not included 100 assays $506.39
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BHE16300024-2 MRPA100KE enzyme included 100 assays $848.57
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