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Home / Assay Kits

Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kits

SKU : BHE16300031

This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA.

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Mfr.No.	HCMV100K, HCMV100KE
Description	Human cytomegalovirus (HCMV) or human betaherpesvirus 5 belongs to the viral family of herpesviruses. HCMV encodes for its own DNA polymerase and uses the host RNA polymerase for the transcription of all of its genes. The HCMV DNA polymerase is a key enzyme in the lytic phase of the infection. The HCMV DNA polymerase assay is based on measurement of the DNA molecules synthesized by the DNA polymerase in the presence of a DNA template and dNTPs. The assay can be performed in a 384-well or 96-well plate format for tests of the enzyme activities of HCMV DNA polymerases and high throughput screening of inhibitors. The Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit (Catalog No. HCMV100K) includes all the assay kit components except the enzyme. It includes 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA.
Drug target assays	Antiviral drug targets

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl. 1.Reaction: The total volume of each reaction mixture is 30 µl including 26.1 µl of H2O, 3 µl of 10 x Buffer, 0.3 µl of 100 x template, 0.3 µl of 100 x RNA polymerase and 0.3 µl of 100 x NTPs. Incubate the reaction mixture at 37°C for 60 min. 2.Detection: Dilute the 2 x fluorescence dye with 50 mM EDTA to make the 1 x dye. Mix 30 µl of the 1 x dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm in 5 min.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300031-1	HCMV100K	enzyme not included	100 assays	$365.40		Add to Cart
BHE16300031-2	HCMV100KE	enzyme included	100 assays	$613.14		Add to Cart

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