HSP90 alpha ELISA Kit

Mfr.No.	SKT-107
Alternative Names	HSP86 ELISA Kit, HSP89A ELISA Kit, HSP90A ELISA Kit, HSP90AA1 ELISA Kit, HSPC1 ELISA Kit, HSPCA ELISA Kit, HsoCAL3 ELISA Kit, HSP90alpha ELISA, HSP86 ELISA Kit ; HSP89A ELISA Kit ; HSP90A ELISA Kit ; HSP90AA1 ELISA Kit ; HSPC1 ELISA Kit ; HSPCA ELISA Kit ; HsoCAL3 ELISA Kit ; HSP90alpha ELISA
Categories	ELISA Kits
Description	Colorimetric detection of HSP90 alpha
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	500
Reactivity	Human, Goat
Scientific Background	HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90- regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now known to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(3). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.
Size	1 Plate ; 1 vial/10 ml ; 2 vials ; 1 vial/ 50 ml ; 1 vial/100 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml
Adr Code	Non-hazardous
Assay Overview	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature, 20-25 °C for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Assay Range	0.44 - 28 ng/ml
Assay Type	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation	Sandwich
Component Number	SKC-107A ; SKC-107B ; SKC-107C ; SKC-107D ; SKC-107E ; SKC-107F ; SKC-107G ; SKC-107H ; SKC-107I ; SKC-107J ; SKC-107K
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	0.44 - 28 ng/ml
Incubation Time	30 minutes
Item	Anti-Hsp90a Immunoassay Plate ; 5X Hsp90a Extraction Reagent ; Recombinant Hsp90a Standard ; Standard and Sample Diluent ; 10X Wash Buffer Concentrate ; Anti-Hsp90a Biotinylated Antibody Concentrate ; Anti-Hsp90a Biotinylated Antibody Diluent ; Streptavidin: HRP Concentrate ; Streptavidin: HRP Diluent ; TMB Substrate ; Stop Solution
Method Type	Cell Lysates, Tissue, Serum
Number Of Samples	40 samples in duplicate
Platform	Microplate
Sensitivity	0.117 ng/ml
Species Reactivity Abbreviations	Hu ; Gt
Species Reactivity Medium	Human ; Goat
Trademark	StressXpress®

Research Area	Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Microplate
Utility: ELISA kit used to quantitate HSP90 alpha concentration in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-107A	Anti-Hsp90a Immunoassay Plate	1 Plate
SKC-107B	5X Hsp90a Extraction Reagent	1 vial/10 ml
SKC-107C	Recombinant Hsp90a Standard	2 vials
SKC-107D	Standard and Sample Diluent	1 vial/ 50 ml
SKC-107E	10X Wash Buffer Concentrate	1 vial/100 ml
SKC-107F	Anti-Hsp90a Biotinylated Antibody Concentrate	1 vial/150 µl
SKC-107G	Anti-Hsp90a Biotinylated Antibody Diluent	1 vial/ 13 ml
SKC-107H	Streptavidin: HRP Concentrate	1 vial/150 µl
SKC-107I	Streptavidin: HRP Diluent	1 vial/ 13 ml
SKC-107J	TMB Substrate	1 vial/ 13 ml
SKC-107K	Stop Solution	1 vial/ 13 ml

Incubation Time: 30 minutes
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 500
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

ELISA kit used to quantitate HSP90 alpha concentration in samples.

Reference	1. Arlander S.J.H., et al. (2003) J Biol Chem. 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem. 59: 157-186. 3. Neckers L, et al. (2002) Trends Mol Med. 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med. 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev. 18: 306-360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med. 217: 420-434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA. 91: 8324- 8328.

Protocols Description	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature, 20-25 °C for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Other Resources 1	Kit Booklet Lot No. BH585999
Other Resources 2	Kit Booklet Lot No. SH585170
Other Resources 3	Kit Booklet Lot No. 1008
Other Resources 4	MSDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900005-1	SKT-107-480	5 X 96 well	$2923		Add to Cart
BHE11900005-2	SKT-107-96	96 well	$688		Add to Cart

HSP90 alpha ELISA Kit

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