HSP70 ELISA Kit

Mfr.No.	SKT-105
Alternative Names	HSP70 1 ELISA Kit, HSP70 2 ELISA Kit, HSP70.1 ELISA Kit, HSP72 ELISA Kit, HSPA1 ELISA Kit, HSPA1A ELISA Kit, HSPA1B ELISA Kit, HSP70 1 ELISA Kit ; HSP70 2 ELISA Kit ; HSP70.1 ELISA Kit ; HSP72 ELISA Kit ; HSPA1 ELISA Kit ; HSPA1A ELISA Kit ; HSPA1B ELISA Kit
Categories	ELISA Kits
Description	Colorimetric detection of HSP70
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	500
Reactivity	Human, Goat, Dog
Scientific Background	HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Size	1 Plate ; 1 vial/10 ml ; 2 vials ; 1 vial/ 50 ml ; 1 vial/100 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml
Adr Code	Non-hazardous
Assay Overview	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard to appropriate wells. 3. Add 50 µL of Pre-Treatment Buffer to all sample wells. 4. Add 50 µL of sample to appropriate wells. 5. Cover plate with Plate Sealer and incubate at 37°C for 2 hours. 6. Wash plate four times with 1X Wash Buffer. 7. Add 100 µL of Detection Antibody Working Solution to each well. 8. Cover plate with Plate Sealer and incubate at 37°C for 2 hours. 9. Wash plate four times with 1X Wash Buffer as described in step 6. 10. Add 100 µL of Streptavidin-HRP Working Solution to each well. 11. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 12. Wash plate four times with 1X Wash Buffer as described in step 6. 13. Add 100 µL of TMB Substrate to each well. 14. Develop the plate in the dark at room temperature for 30 minutes. 15. Stop reaction by adding 100 µL of Stop Solution to each well. 16. Measure absorbance on a plate reader at 450 nm.
Assay Range	0.781 - 50 ng/ml
Assay Type	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation	Sandwich
Component Number	SKC-105A ; SKC-105B ; SKC-105C ; SKC-105D ; SKC-105E ; SKC-105F ; SKC-105G ; SKC-105H ; SKC-105I ; SKC-105J ; SKC-105K ; SKC-105L
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	0.781 - 50 ng/ml
Incubation Time	30 minutes
Item	Anti-Hsp70 Immunoassay Plate ; 5X Hsp70 Extraction Reagent ; Recombinant Hsp70 Standard ; Standard and Sample Diluent ; 10X Wash Buffer Concentrate ; Anti-Hsp70 Biotinylated Antibody Concentrate ; Anti-Hsp70 Biotinylated Antibody Diluent ; Streptavidin: HRP Concentrate ; Streptavidin: HRP Diluent ; TMB Substrate ; Stop Solution ; Pre-treatment Buffer
Method Type	Cell Lysates, Tissue
Number Of Samples	40 samples in duplicate
Platform	Microplate
Sensitivity	0.18 ng/ml
Species Reactivity Abbreviations	Hu ; Gt ; Dg
Species Reactivity Medium	Human ; Goat ; Dog
Trademark	StressXpress®

Research Area	Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Microplate
Utility: ELISA kit used to quantitate HSP70 concentration in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-105A	Anti-Hsp70 Immunoassay Plate	1 Plate
SKC-105B	5X Hsp70 Extraction Reagent	1 vial/10 ml
SKC-105C	Recombinant Hsp70 Standard	2 vials
SKC-105D	Standard and Sample Diluent	1 vial/ 50 ml
SKC-105E	10X Wash Buffer Concentrate	1 vial/100 ml
SKC-105F	Anti-Hsp70 Biotinylated Antibody Concentrate	1 vial/150 µl
SKC-105G	Anti-Hsp70 Biotinylated Antibody Diluent	1 vial/ 13 ml
SKC-105H	Streptavidin: HRP Concentrate	1 vial/150 µl
SKC-105I	Streptavidin: HRP Diluent	1 vial/ 13 ml
SKC-105J	TMB Substrate	1 vial/ 13 ml
SKC-105K	Stop Solution	1 vial/ 13 ml
SKC-105L	Pre-treatment Buffer	1 vial/ 13 ml

Incubation Time: 30 minutes
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 500
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

ELISA kit used to quantitate HSP70 concentration in samples.

Reference	1. Zho J. (1998) Cell. 94: 471-480. 2. Boorstein W.R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al. (1993) Mol. Cell. Biol. 13(2): 869-876. 8. Prapapanich V., et al., (1996) Mol. Cell. Biol. 16(11): 6200-6207. 9. Fernandez-Funez et al., (2000) Nature. 408(6808): 101-106.

Protocols Description	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard to appropriate wells. 3. Add 50 µL of Pre-Treatment Buffer to all sample wells. 4. Add 50 µL of sample to appropriate wells. 5. Cover plate with Plate Sealer and incubate at 37°C for 2 hours. 6. Wash plate four times with 1X Wash Buffer. 7. Add 100 µL of Detection Antibody Working Solution to each well. 8. Cover plate with Plate Sealer and incubate at 37°C for 2 hours. 9. Wash plate four times with 1X Wash Buffer as described in step 6. 10. Add 100 µL of Streptavidin-HRP Working Solution to each well. 11. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 12. Wash plate four times with 1X Wash Buffer as described in step 6. 13. Add 100 µL of TMB Substrate to each well. 14. Develop the plate in the dark at room temperature for 30 minutes. 15. Stop reaction by adding 100 µL of Stop Solution to each well. 16. Measure absorbance on a plate reader at 450 nm.
Other Resources 1	Kit Booklet Lot No. 121107
Other Resources 2	MSDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900001-1	SKT-105-480	5 X 96 well	$2432		Add to Cart
BHE11900001-2	SKT-105-96	96 well	$572		Add to Cart

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