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Home / Assay Kits

Glycogen Fluorometric Assay Kit

SKU : BHE10308176

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	E-BC-F040
Assay Time	50min
Assay Type	QuantitatIVe
Inter-cv (%)	6.6
Intra-cv (%)	3.4
Manual	Data Sheet
Sensitivity	0.06 ug/mL
Test Principle	Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oXidase to produce hydrogen peroXide. In the presence of the peroXidase, hydrogen peroXide be oXidized to produce the fluorescence substrate. The fluorescence intensity at the eXcitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.

Research Area	Glycolysis & Carbohydrates

Dilutions	The optimal sampling volume are different for differentspecies, the SOD also are different for different samples. It is recommended totake 2~3 samples to do a pre-experiment, diluting a series of diluent anddetermine the dilution factor when the SOD inhibition ratio is 15%~55% ( the optimal inhibition ratio is the range of 25%~45%. ) before formal experiment.The recommended dilution factor for different samples is as follows (for referenceonly): Sample type Dilution factor Thevolumeofsample, HepG2 supeatant 1 50 uL, HepG2 cell 8-10 25 uL, Mouse serum 3-5 20 uL, 10% Mouse liver tissue homogenate 40-60 20 uL, 10% Rat kidney tissue homogenate 15 -20 20 uL, Human urine 1 25 uL. Note: The diluent is normalsaline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Storage	-20°C
Valid Period	6 Months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10308176	E-BC-F040	96T	$425		Add to Cart

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