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Home / Assay Kits

Glucose Uptake Fluorometric Assay Kit

SKU : BHE10308177

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	E-BC-F041
Assay Time	100 min
Assay Type	QuantitatIVe
Manual	Data Sheet
Sensitivity	0.02 nmol/uL
Test Principle	2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the eXcitation wavelength of 530 nm and the emission wavelength of 590 nm.

Research Area	Others

Dilutions	The optimal sampling volume are different for different species, the SODalso are different for different samples. It is recommended to take 2~3 samplesto do a pre-experiment, diluting a series of diluent and determine the dilutionfactor when the SOD inhibitionratio is 25%~65% ( the optimal inhibition ratio is the range of 40%~60%. ) before formal experiment.The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, Human serum 3-5, Rat serum 20-30, Urine Human hydrothorax 2, Cell culture supernatant 2-3, 10% Rat liver tissue homogenate 340-370, 10% Rat heart tissue homogenate 80-100, 10% Rat kidney tissue homogenate 100-120, 10% Rat brain tissue homogenate 50-100, 10% Plant tissue homogenate 5-10, HepG2 cells (3 mgprot/mL) 30-40. Note: Thediluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Storage	-20°C
Valid Period	6 Months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10308177	E-BC-F041	96T	$780		Add to Cart

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