Gel-shift single-strand DNA binding assay. Single-strand DNA-binding protein (SSB) binds to single-stranded DNA (ssDNA) molecules or a single-stranded region of a double strand-DNA in a biological process such as DNA replication, recombination, or repair. For example, DNA helicase unwinds double-strand DNA to form single-strand DNA. E. coli SSB forms homotetramer; each subunit has a molecular weight of 19 kDa. The Gel Shift Single-strand DNA Binding Assay is designed for analysis of SSB DNA-binding activity using a conventional acrylamide electrophoresis. The SSB DNA-binding activity is visualized by the DNA band shift. The SSB-bound DNA migrates slower than the free DNA. This product includes 150 ul of 10 x Buffer, 150 ul of 10 x ssDNA (a 45-mer single-strand DNA) and 100 ul of 4 x gel loading buffer.
Binding and separation
Binding
Reviews of Gel Shift Single-strand DNA Binding Assay Kit