Sensitive and accurate. Detection limit of 0.02 uM and linearity up to 1 uM NAD+/NADH in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
F530/585nm
Detection Principle
0.02 uM
Shelf Life
6 months
Specifity
For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Reference
Chaurasiya, A., et al. (2021). Pathogen induced subversion of NAD+ metabolism mediating host cell death: a target for development of chemotherapeutics. Cell Death Discovery 7(1): 10. Assay: NAD and NADH in microbial cells.
Joe, Y., et al. (2020). Cross-talk between CD38 and TTP is essential for resolution of inflammation during microbial sepsis. Cell Reports 30(4): 1063-1076.e5. Assay: NAD and NADH in mouse cells.
Zhang, M., et al. (2020). Dysregulated metabolic pathways in age-related macular degeneration. Scientific Reports 10(1): 2464. Assay: NAD and NADH in human cells.
Liemburg-Apers, Dania C., et al (2016). Acute stimulation of glucose influx upon mitoenergetic dysfunction requires LKB1, AMPK, Sirt2 and mTOR-RAPTOR. J Cell Sci 129.23: 4411-4423. Assay: NAD/NADH in mouse cells.
Kim, Ha-Neui, et al. (2015) Sirtuin1 suppresses osteoclastogenesis by deacetylating FoxOs. Molecular Endocrinology 29.10: 1498-1509. Assay: NAD/NADH in mice.
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