New and improved. Total assay time reduced from the standard 9 hours to 5 hours.Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary.Accurate. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations.
Intended Use
Cell-Based ELISA (FL530/585 nm,360/450 nm)
Shelf Life
6 months
Specifity
Determination of RB(T821/826) phosphorylation status in whole cells.Evaluation of pathway modulation by activators, inhibitors, siRNA etc.