Safe. Non-radioactive assay.Sensitive and accurate. Linear detection range of 1 - 150 uM bile acids. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can bereadily automated on HTS liquid handling systems for processing thousands of samples per day.
Intended Use
FL530/585nm
Detection Principle
1 µM
Shelf Life
6 months
Specifity
For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
Reference
Ommati, M. M., et al (2020) Oral administration of thiol-reducing agents mitigates gut barrier disintegrity and bacterial lipopolysaccharide translocation in a rat model of biliary obstruction. Current Research in Pharmacology and Drug Discovery, 1, 10-18. Assay: Bile Acid in rats serum.
Ommati, M. M., Farshad, O., Niknahad, H., Arabnezhad, M. R., Azarpira, N., Mohammadi, H. R., Haghnegahdar, M., Mousavi, K., Akrami, S., Jamshidzadeh, A., & Heidari, R. (2019). Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress. Toxicology Letters, 316, 60-72. Assay: Bile Acid in rats serum, ovary and testes tissue.
Sano, M., et al (2020). Increase in the lipopolysaccharide activity and accumulation of gram-negative bacteria in the stomach with low acidity. Clinical and Translational Gastroenterology, 11(7). Assay: Bile Acid in human gastric fluid.
Ommati, M. M.,et al (2020). N-acetyl cysteine treatment mitigates biomarkers of oxidative stress in different tissues of bile duct ligated rats. Stress (Amsterdam, Netherlands), 1-16. Assay: Bile Acid in rats serum and tissue.
Igarashi, Muneki, et al (2017). Alteration in the gastric microbiota and its restoration by probiotics in patients with functional dyspepsia. BMJ open gastroenterology 4.1: e000144. Assay: Bile Acid in human gastric fluid.
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