Sensitive. Can measure pAMPK modulation in as little as 500 cells/well.Simple and convenient. No cell lysis necessary, cells can be cultured for several days.
Intended Use
FL360/450nm,530/585nm
Shelf Life
6 months
Specifity
For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.
Reference
Cao, J. et al (2020). 6PGD Upregulation is Associated with Chemo-and Immuno-Resistance of Renal Cell Carcinoma via AMPK Signaling-Dependent NADPH-Mediated Metabolic Reprograming. The American journal of the medical sciences, 360(3), 279-286. Assay: Phosphorylated AMPK in human renal carcinoma cells.
Chen, Hu, et al (2019). 6PGD inhibition sensitizes hepatocellular carcinoma to chemotherapy via AMPK activation and metabolic reprogramming. Biomedicine & Pharmacotherapy 111: 1353-1358. Assay: AMPK phosphorylation in human hepatic cell.
Tsuda, Yuichi, et al (2017). "Trypsin-Treated beta-Lactoglobulin Improves Glucose Tolerance in C57BL/6 Mice by Enhancing AMPK Activation and Glucose Uptake in Hepatocytes." Biological and Pharmaceutical Bulletin 40.11: 1917-1922. Assay: AMPK phosphorylation in human hepatocytes.
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