Fast and sensitive. Linear detection range (20 uL sample):50 to 1000 uM urea in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Intended Use
OD340nm
Detection Principle
50 uM
Shelf Life
6 months
Specifity
For quantitative enzymatic determination of urea.
Reference
Duarte, N. T. et al (2020). Prevalence of sublingual varices in patients with cirrhosis and the correlation with nitrogen compounds. Oral surgery, oral medicine, oral pathology and oral radiology, 129(1), 39-44. Assay: urea in human saliva and blood.
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