Fast and sensitive. Use 20 µL sample. Linear detection range 10 to 400 µM for colorimetric assays and 2 to 40 µM for fluorimetric assays.Convenient. The procedure involves adding a single working reagent, and reading the absorbance or fluorescence after 30 minutes. Room temperature assay. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples.
Reference
Ceperuelo-Mallafre, V et al (2019). Preoperative circulating succinate levels as a biomarker for diabetes remission after bariatric surgery. Diabetes Care, 42(10), 1956-1965. Assay: Succinate in human serum.
Astiarraga, B et al (2020). Impaired succinate response to a mixed meal in obesity and type 2 diabetes is normalized after metabolic surgery. Diabetes Care, 43(10), 2581-2587. Assay: Succinate in human plasma.
Serena, C., Ceperuelo-Mallafre, V., Keiran, N., Queipo-Ortuno, M. I., Bernal, R., Gomez-Huelgas, R., & Moya, A. (2018). Elevated circulating levels of succinate in human obesity are linked to specific gut microbiota. The ISME journal, 12(7), 1642. Assay: Succinate in human serum.
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