| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | succinate in food, beverage, agricultural products, and other biological samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of succinate (succinic acid) in food, beverage, agricultural products, and other biological samples. The assay uses OD570nm, FL530/585nm for signal readout. Compatible sample input includes succinate in food, beverage, agricultural products, and other biological samples. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD570nm, FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes succinate in food, beverage, agricultural products, and other biological samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of Colorimetric assay: 10 µM; Fluorimetric assay: 2 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use a 20 µL sample. Linear detection range from 10 to 400 µM for colorimetric assays and 2 to 40 µM for fluorimetric assays.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading the absorbance or fluorescence after 30 minutes. Room temperature assay. No 37°C heater is needed; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of succinate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
SUCCINATE, or succinic acid, can be found in all plants and animal tissues. It is an intermediate in the citric acid cycle and plays an important role in intracellular energy generation. Succinate is widely used as a flavoring agent in the food, beverage, and pharmaceutical industries due to its low toxicity.BioAssay Systems′ succinate assay provides a simple, one-step assay for measuring succinate. In this assay, succinate is converted to pyruvate which reacts with specific reagents and dye to form a colored product. The color intensity at 570 nm or fluorescence at Fex/em = 530/585 nm of the reaction product is directly proportional to the succinate concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit(s): Colorimetric: 10 µM; / Fluorescent: 2 µM. Additional source wording: Colorimetric assay: 10 µM; Fluorimetric assay: 2 µM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify succinate in succinate in food, beverage, agricultural by OD570 nm, FL530/585 nm readout.
- Compare treatment or phenotype groups using matched succinate in food, beverage, agricultural handling.
- Monitor time-course or pre/post changes in succinate in food, beverage, agricultural across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the assay kit work in particular species? And what samples have you tested?
Yes, our assays work in all species. We have tested in red wine, soy sauce and urine samples.
I don’t have the correct wavelength filter, will the assay still work?
Typically if you are within ±20 nm the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.
I don’t have a 96-well plate, will the assay kit work in a 384 well plate, cuvette, etc?
Yes, the assay can be used in any standard plate and cuvette. Simply adjust the total reaction volume to your container. For 384 well use 50 µL, use a narrow cuvette for 500 µL.
How do I prepare cell or tissue samples for assays?
Cell Samples. Suspend about two million (2 x 106) harvested cells in 400 µL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homeginer on ice) or by sonication (preferably performed in an ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
6
g
Tissue Samples. Start with 20-100 mg tissue, add 200- 1000 µL ice-cold PBS. Lysis can be achieved by homogenization (10-20 passes in a Dounce homeginer on ice) or by sonication (preferably performed in an ice-water bath). The degree of tissue lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
g
Urine sample: no dilution necessary for colorimetric assays; 1:10 in H2O for fluorimetric assays.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided.
Do I need to use a standard or standard curve with each assay run?
Yes, standard curve should be performed with each fluorimetric assay run. Internal standard should be included with each colorimetric assay run.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Preoperative circulating succinate levels as a biomarker for diabetes remission after bariatric surgery
Ceperuelo-Mallafre, V et al (2019). Preoperative circulating succinate levels as a biomarker for diabetes remission after bariatric surgery. Diabetes Care, 42(10), 1956-1965. Assay: Succinate in human serum.
Impaired succinate response to a mixed meal in obesity and type 2 diabetes is normalized after metabolic surgery
Astiarraga, B et al (2020). Impaired succinate response to a mixed meal in obesity and type 2 diabetes is normalized after metabolic surgery. Diabetes Care, 43(10), 2581-2587. Assay: Succinate in human plasma.
Elevated circulating levels of succinate in human obesity are linked to specific gut microbiota
Serena, C., Ceperuelo-Mallafre, V., Keiran, N., Queipo-Ortuno, M. I., Bernal, R., Gomez-Huelgas, R., & Moya, A. (2018). Elevated circulating levels of succinate in human obesity are linked to specific gut microbiota. The ISME journal, 12(7), 1642. Assay: Succinate in human serum.
